Zinc Alpha 2 Glycoprotein Recombinant Rabbit Monoclonal Antibody [JE35-38]
Catalog# HA722298
Zinc Alpha 2 Glycoprotein Recombinant Rabbit Monoclonal Antibody [JE35-38]
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WB
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Human
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unconjugated
Overview
Product Name
Zinc Alpha 2 Glycoprotein Recombinant Rabbit Monoclonal Antibody [JE35-38]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human Zinc Alpha 2 Glycoprotein aa 1-100 / 298.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 34 kDa
Positive Control
Human serum lysate, Human plasma lysate.
Conjugation
unconjugated
Clone Number
JE35-38
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Zinc-alpha-2-glycoprotein is a protein that in humans is encoded by the AZGP1 gene. This gene expresses a soluble protein that stimulates lipolysis, induces a reduction in body fat in mice, is associated with the cachexia related to cancer, and is known to be expressed in secretory cells of lung epithelium. In 2009, it was found that smoking increases expression of this gene, which is why smoking cessation leads to weight gain. Zinc-alpha-2-glycoprotein levels also rise with onset of diabetes 2, which accounts for weight loss thereafter.
Background References
1. Alenad AM et al. Associations of zinc-alpha-2-glycoprotein with metabolic syndrome and its components among adult Arabs. Sci Rep. 2022 Mar
2. Verma S et al. Zinc Alpha-2-Glycoprotein (ZAG/AZGP1) secreted by triple-negative breast cancer promotes tumor microenvironment fibrosis. bioRxiv [Preprint]. 2024 Mar
Subcellular Location
Secreted.
UNIPROT
Synonyms
Alpha 2 glycoprotein 1 zinc antibody
Alpha 2 glycoprotein 1 zinc binding antibody
Alpha 2 glycoprotein zinc antibody
Azgp1 antibody
ZA2G antibody
ZA2G_HUMAN antibody
ZAG antibody
Zinc alpha 2 glycoprotein precursor antibody
Zinc-alpha-2-glycoprotein antibody
Zn alpha 2 glycoprotein antibody
ExpandImages
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Western blot analysis of Zinc Alpha 2 Glycoprotein on different lysates with Rabbit anti-Zinc Alpha 2 Glycoprotein antibody (HA722298) at 1/1,000 dilution.
Lane 1: Human serum lysate
Lane 2: Human plasma lysate
Lysates/proteins at 15 µg/Lane.
Predicted band size: 34 kDa
Observed band size: 41 kDa
Exposure time: 10 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722298) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"