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C1s Recombinant Rabbit Monoclonal Antibody [JE61-95]

Usd: 385

Specification

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Catalog# HA722292

C1s Recombinant Rabbit Monoclonal Antibody [JE61-95]

Application

  • WB

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

C1s Recombinant Rabbit Monoclonal Antibody [JE61-95]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human C1s aa 1-100.

Species Reactivity

Human

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 77 kDa

Positive Control

A549 cell lysates, human plasma lysates, human liver tissue, human liver cancer tissue.

Conjugation

unconjugated

Clone Number

JE61-95

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

Target

Function

Complement component 1s (EC 3.4.21.42, C1 esterase, activated complement C1s, complement C overbar 1r, C1s) is a protein involved in the complement system. C1s is part of the C1 complex. In humans, it is encoded by the C1S gene. C1s cleaves C4 and C2, which eventually leads to the production of the classical pathway C3-convertase.

Background References

1. Ye J et al. Complement C1s as a diagnostic marker and therapeutic target: Progress and propective. Front Immunol. 2022 Oct

2. Moore DC et al. Sutimlimab: A Complement C1s Inhibitor for the Management of Cold Agglutinin Disease-Associated Hemolysis. Ann Pharmacother. 2023 Aug

Subcellular Location

Blood microparticle, extracellular region, extracellular space.

UNIPROT

SwissProt: P09871 Human

Synonyms

Basic proline rich peptide IB 1 antibody

C1 esterase antibody

C1S antibody

C1S_HUMAN antibody

Complement C1s subcomponent antibody

Complement C1s subcomponent heavy chain antibody

Complement C1s subcomponent light chain antibody

Complement component 1 s subcomponent antibody

Complement component 1 subcomponent s antibody

FLJ44757 antibody

Images

  • Western blot analysis of C1s on A549 cell lysates with Rabbit anti-C1s antibody (<a href="/products/HA722292" style="font-weight: bold;text-decoration: underline;">HA722292</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 77 kDa<br />Observed band size: 90 kDa<br /><br />Exposure time: 1 minute 50 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722292" style="font-weight: bold;text-decoration: underline;">HA722292</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of C1s on A549 cell lysates with Rabbit anti-C1s antibody (HA722292) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 77 kDa
    Observed band size: 90 kDa

    Exposure time: 1 minute 50 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722292) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of C1s on human plasma lysates with Rabbit anti-C1s antibody (<a href="/products/HA722292" style="font-weight: bold;text-decoration: underline;">HA722292</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 77 kDa<br />Observed band size: 90 kDa<br /><br />Exposure time: 2 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722292" style="font-weight: bold;text-decoration: underline;">HA722292</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of C1s on human plasma lysates with Rabbit anti-C1s antibody (HA722292) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 77 kDa
    Observed band size: 90 kDa

    Exposure time: 2 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722292) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-C1s antibody (<a href="/products/HA722292" style="font-weight: bold;text-decoration: underline;">HA722292</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722292" style="font-weight: bold;text-decoration: underline;">HA722292</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver tissue with Rabbit anti-C1s antibody (HA722292) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722292) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-C1s antibody (<a href="/products/HA722292" style="font-weight: bold;text-decoration: underline;">HA722292</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722292" style="font-weight: bold;text-decoration: underline;">HA722292</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human liver cancer tissue with Rabbit anti-C1s antibody (HA722292) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722292) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"