CA12 Recombinant Rabbit Monoclonal Antibody [PSH05-49]
Catalog# HA722287
CA12 Recombinant Rabbit Monoclonal Antibody [PSH05-49]
-
WB
-
IF-Cell
-
FC
-
Human
-
unconjugated
Overview
Product Name
CA12 Recombinant Rabbit Monoclonal Antibody [PSH05-49]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human CA12 aa 1-301 / 354.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 40 kDa
Positive Control
A549 cell lysate, MCF7 cell lysate, A549.
Conjugation
unconjugated
Clone Number
PSH05-49
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IF-Cell
-
1:500
-
FC
-
1:1,000
Target
Function
Carbonic anhydrase 12 is an enzyme that in humans is encoded by the CA12 gene. Carbonic anhydrases (CAs) are a large family of zinc metalloenzymes that catalyze the reversible hydration of carbon dioxide. They participate in a variety of biological processes, including respiration, calcification, acid-base balance, bone resorption, and the formation of aqueous humor, cerebrospinal fluid, saliva, and gastric acid. This gene product is a type I membrane protein that is highly expressed in normal tissues, such as kidney, colon and pancreas, and has been found to be overexpressed in 10% of clear cell renal carcinomas. Two transcript variants encoding different isoforms have been identified for this gene.
Background References
1. Du Y et al. Overexpressed CA12 has prognostic value in pancreatic cancer and promotes tumor cell apoptosis via NF-kappaB signaling. J Cancer Res Clin Oncol. 2021 May
2. Shen M et al. Correlation between CA12 and TFF3 and their prediction value of neoadjuvant chemotherapy response in breast cancer. J Clin Pharm Ther. 2022 May
Subcellular Location
Membrane.
UNIPROT
Synonyms
CA 12 antibody
CA XII antibody
CA-XII antibody
CA12 antibody
CAH12_HUMAN antibody
Carbonate dehydratase XII antibody
Carbonic anhydrase 12 antibody
Carbonic anhydrase XII antibody
Carbonic dehydratase antibody
CAXII antibody
ExpandImages
-
Western blot analysis of CA12 on different lysates with Rabbit anti-CA12 antibody (HA722287) at 1/2,000 dilution.
Lane 1: A549 cell lysate (15 µg/Lane)
Lane 2: MCF7 cell lysate (15 µg/Lane)
Predicted band size: 40 kDa
Observed band size: 45 kDa
Exposure time: 54 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722287) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of A549 cells labeling CA12 with Rabbit anti-CA12 antibody (HA722287) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CA12 antibody (HA722287) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of A549 cells labeling CA12.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722287, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
CA12 Rabbit Polyclonal Antibody
Application: WB,IHC-P,IF-Cell,FC
Reactivity: Human,Mouse,Rat
Conjugate: unconjugated
