DLX3 Recombinant Rabbit Monoclonal Antibody [JE64-45]
Catalog# HA722281
DLX3 Recombinant Rabbit Monoclonal Antibody [JE64-45]
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WB
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IF-Cell
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IHC-P
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FC
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IF-Tissue
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Human
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unconjugated
Overview
Product Name
DLX3 Recombinant Rabbit Monoclonal Antibody [JE64-45]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human DLX3 aa 238-287 / 287.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, IHC-P, FC, IF-Tissue
Target Molecular Weight
Predicted band size: 32 kDa
Positive Control
JAR cell lysates, JAR, human skin tissue.
Conjugation
unconjugated
Clone Number
JE64-45
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
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IHC-P
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1:1,000
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FC
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1:1,000
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IF-Tissue
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1:50-1:200
Target
Function
Homeobox protein DLX-3 is a protein that in humans is encoded by the DLX3 gene. Dlx3 is a crucial regulator of hair follicle differentiation and cycling. Dlx3 transcription is mediated through Wnt, and colocalization of Dlx3 with phospho-SMAD1/5/8 is involved in the regulation of transcription by BMP signaling. Dlx3 transcription is also induced by BMP-2 through transactivation with SMAD1 and SMAD4. Many vertebrate homeo box-containing genes have been identified on the basis of their sequence similarity with Drosophila developmental genes. Members of the Dlx gene family contain a homeobox that is related to that of Distal-less (Dll), a gene expressed in the head and limbs of the developing fruit fly. The Distal-less (Dlx) family of genes comprises at least 6 different members, DLX1-DLX6. This gene is located in a tail-to-tail configuration with another member of the gene family on the long arm of chromosome 17.
Background References
1. Zheng H et al. Dlx3 Ubiquitination by Nuclear Mdm2 Is Essential for Dentinogenesis in Mice. J Dent Res. 2022 Aug
2. Bajpai D et al. Loss of DLX3 tumor suppressive function promotes progression of SCC through EGFR-ERBB2 pathway. Oncogene. 2021 May
Subcellular Location
Nucleus, Cytoplasm.
UNIPROT
Synonyms
AI4 antibody
Distal less homeo box 3 antibody
DLX 3 antibody
Dlx3 antibody
DLX3 distalless homeobox 3 antibody
DLX3_HUMAN antibody
Homeobox protein DLX 3 antibody
Homeobox protein DLX-3 antibody
Homeobox protein Dlx3 antibody
TDO antibody
Images
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Western blot analysis of DLX3 on JAR cell lysates with Rabbit anti-DLX3 antibody (HA722281) at 1/1,000 dilution.
Lysates/proteins at 20 µg/Lane.
Predicted band size: 32 kDa
Observed band size: 38 kDa
Exposure time: 20 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722281) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of JAR cells labeling DLX3 with Rabbit anti-DLX3 antibody (HA722281) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-DLX3 antibody (HA722281) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI. Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human skin tissue with Rabbit anti-DLX3 antibody (HA722281) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722281) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX. -
Flow cytometric analysis of JAR cells labeling DLX3.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722281, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"