GPR56 Recombinant Rabbit Monoclonal Antibody [PSH05-43]
Catalog# HA722273
GPR56 Recombinant Rabbit Monoclonal Antibody [PSH05-43]
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WB
-
IHC-P
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Human
-
unconjugated
Overview
Product Name
GPR56 Recombinant Rabbit Monoclonal Antibody [PSH05-43]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human GPR56 aa 26-402.
Species Reactivity
Human
Validated Applications
WB, IHC-P
Target Molecular Weight
Predicted band size: 78 kDa
Positive Control
A-172 cell lysate, U-87 MG cell lysate, T-47D cell lysate, human brain tissue lysate, human glioma tissue.
Conjugation
unconjugated
Clone Number
PSH05-43
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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IHC-P
-
1:1,000
Target
Function
G protein-coupled receptor 56 also known as TM7XN1 is a protein encoded by the ADGRG1 gene. GPR56 is a member of the adhesion GPCR family. Adhesion GPCRs are characterized by an extended extracellular region often possessing N-terminal protein modules that is linked to a TM7 region via a domain known as the GPCR-Autoproteolysis INducing (GAIN) domain. GPR56 is expressed in liver, muscle, tendon, neural, and cytotoxic lymphoid cells in human as well as in hematopoietic precursor, muscle, and developing neural cells in the mouse. GPR56 has been shown to have numerous role in cell guidance/adhesion as exemplified by its roles in tumour inhibition and neuron development. More recently it has been shown to be a marker for cytotoxic T cells and a subgroup of Natural killer cells.
Background References
1. Singh AK et al. The role of GPR56/ADGRG1 in health and disease. Biomed J. 2021 Oct
2. Ng KF et al. Role of ADGRG1/GPR56 in Tumor Progression. Cells. 2021 Nov
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
7 transmembrane protein with no EGF like N terminal domains 1 antibody
BFPP antibody
DKFZp781L1398 antibody
EGF TM7 like antibody
G protein coupled receptor 56 antibody
G-protein coupled receptor 56 antibody
GPR 56 antibody
Gpr56 antibody
GPR56_HUMAN antibody
Polymicrogyria bilateral frontoparietal antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of GPR56 on different lysates with Rabbit anti-GPR56 antibody (HA722273) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.
Lane 1: A-172 cell lysate
Lane 2: U-87 MG cell lysate
Lane 3: T-47D cell lysate
Lane 4: Human liver tissue lysate (negative)
Lane 5: Human brain tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 55-70 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722273) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human glioma tissue with Rabbit anti-GPR56 antibody (HA722273) at 1/200 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722273) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"