ADRM1 Recombinant Rabbit Monoclonal Antibody [PSH05-41]
Catalog# HA722270
ADRM1 Recombinant Rabbit Monoclonal Antibody [PSH05-41]
-
WB
-
IF-Cell
-
FC
-
IP
-
Human
-
Mouse
-
Rat
-
Green monkey
-
unconjugated
Overview
Product Name
ADRM1 Recombinant Rabbit Monoclonal Antibody [PSH05-41]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human ADRM1 aa 258-407 / 407.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IF-Cell, FC, IP
Target Molecular Weight
Predicted band size: 42 kDa
Positive Control
HEK-293 cell lysate, HeLa cell lysate, SK-MEL-28 cell lysate, MCF7 cell lysate, Ramos cell lysate, Raji cell lysate, K-562 cell lysate, U-2 OS cell lysate, SW620 cell lysate, COS-1 cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, C6 cell lysate, SK-MEL-28, NIH/3T3, PC-12.
Conjugation
unconjugated
Clone Number
PSH05-41
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IF-Cell
-
1:500
-
FC
-
1:1,000
-
IP
-
1-2μg/sample
Target
Function
Proteasomal ubiquitin receptor ADRM1 is a protein that in humans is encoded by the ADRM1 gene. As the degradation machinery that is responsible for ~70% of intracellular proteolysis,[13] proteasome complex (26S proteasome) plays a critical roles in maintaining the homeostasis of cellular proteome. Accordingly, misfolded proteins and damaged protein need to be continuously removed to recycle amino acids for new synthesis; in parallel, some key regulatory proteins fulfill their biological functions via selective degradation; furthermore, proteins are digested into peptides for MHC class I antigen presentation. To meet such complicated demands in biological process via spatial and temporal proteolysis, protein substrates have to be recognized, recruited, and eventually hydrolyzed in a well controlled fashion. Thus, 19S regulatory particle pertains a series of important capabilities to address these functional challenges. To recognize protein as designated substrate, 19S complex has subunits that are capable to recognize proteins with a special degradative tag, the ubiquitinylation.
Background References
1. Zhu M et al. ADRM1/RPN13 attenuates cartilage extracellular matrix degradation via enhancing UCH37-mediated ALK5 deubiquitination. Int J Biol Macromol. 2023 Aug
2. Liang YC et al. ADRM1 as a therapeutic target in hepatocellular carcinoma. Kaohsiung J Med Sci. 2021 Jan
Subcellular Location
Cytoplasm, Nucleus.
Synonyms
110 kDa cell membrane glycoprotein antibody
adhesion regulating molecule 1 antibody
Adhesion-regulating molecule 1 antibody
ADRM 1 antibody
Adrm1 antibody
ADRM1_HUMAN antibody
ARM 1 antibody
ARM-1 antibody
ARM1 antibody
Gp110 antibody
ExpandImages
-
Western blot analysis of ADRM1 on different lysates with Rabbit anti-ADRM1 antibody (HA722270) at 1/2,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: SK-MEL-28 cell lysate (20 µg/Lane)
Lane 4: MCF7 cell lysate (20 µg/Lane)
Lane 5: Ramos cell lysate (20 µg/Lane)
Lane 6: Raji cell lysate (20 µg/Lane)
Lane 7: K-562 cell lysate (20 µg/Lane)
Lane 8: U-2 OS cell lysate (20 µg/Lane)
Lane 9: SW620 cell lysate (20 µg/Lane)
Lane 10: COS-1 cell lysate (20 µg/Lane)
Lane 11: NIH/3T3 cell lysate (20 µg/Lane)
Lane 12: RAW264.7 cell lysate (20 µg/Lane)
Lane 13: PC-12 cell lysate (20 µg/Lane)
Lane 14: C6 cell lysate (20 µg/Lane)
Predicted band size: 42 kDa
Observed band size: 42 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722270) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of SK-MEL-28 cells labeling ADRM1 with Rabbit anti-ADRM1 antibody (HA722270) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ADRM1 antibody (HA722270) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of NIH/3T3 cells labeling ADRM1 with Rabbit anti-ADRM1 antibody (HA722270) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ADRM1 antibody (HA722270) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of PC-12 cells labeling ADRM1 with Rabbit anti-ADRM1 antibody (HA722270) at 1/500 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-ADRM1 antibody (HA722270) at 1/500 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of SK-MEL-28 cells labeling ADRM1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722270, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of NIH/3T3 cells labeling ADRM1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722270, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
Flow cytometric analysis of PC-12 cells labeling ADRM1.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA722270, 1/1,000) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black). -
ADRM1 was immunoprecipitated from 0.2 mg HEK-293 cell lysate with HA722270 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722270 at 1/1,000 dilution. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.
Lane 1: HEK-293 cell lysate (input)
Lane 2: HA722270 IP in HEK-293 cell lysate
Lane 3: Rabbit IgG instead of HA722270 in HEK-293 cell lysate
Blocking/Dilution buffer: 5% NFDM/TBST
Exposure time: 3 minutes; ECL: K1802
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"