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Mouse IL-4 Recombinant Rabbit Monoclonal Antibody [PSH05-33] - BSA and Azide free (Capture)

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Specification

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Catalog# HA722262

Mouse IL-4 Recombinant Rabbit Monoclonal Antibody [PSH05-33] - BSA and Azide free (Capture)

Application

  • ELISA(Cap)

Reactivity

  • Mouse

Pair: Capture
Pair: Detector
Pair: Protein
Range

  • 8.2-2,000 pg/mL

PI

  • 7.59

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Mouse IL-4 Recombinant Rabbit Monoclonal Antibody [PSH05-33] - BSA and Azide free (Capture)

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Mouse IL-4 aa 21-140 (P07750).

Species Reactivity

Mouse

Validated Applications

ELISA(Cap)

Molecular Weight

Predicted band size: 16 kDa

Positive Control

Recombinant protein within Mouse IL-4 aa 21-140 (P07750).

Conjugation

unconjugated

Clone Number

PSH05-33

Product Features

Form

Liquid

Concentration

Storage Instructions

Store at +4℃ after thawing. Aliquot store at -20℃. Avoid repeated freeze / thaw cycles.

Storage Buffer

PBS (pH7.4).

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • ELISA(Cap)

  • Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal[PSH05-34] to Mouse IL-4 (Detector) (HA722263) and recombinant standard Mouse IL-4 protein (HA210741). The reference range value is 8.2-2,000 pg/mL.

Target

Function

The interleukin 4 (IL4, IL-4) is a cytokine that induces differentiation of naive helper T cells (Th0 cells) to Th2 cells. Upon activation by IL-4, Th2 cells subsequently produce additional IL-4 in a positive feedback loop. IL-4 is produced primarily by mast cells, Th2 cells, eosinophils and basophils. It is closely related and has functions similar to IL-13. Interleukin 4 has many biological roles, including the stimulation of activated B cell and T cell proliferation, and the differentiation of B cells into plasma cells. It is a key regulator in humoral and adaptive immunity. IL-4 induces B cell class switching to IgE, and up-regulates MHC class II production. IL-4 decreases the production of Th1 cells, macrophages, IFNγ, and dendritic cells IL-12. Overproduction of IL-4 is associated with allergies.

Background References

1. Baxter EW et al. Standardized protocols for differentiation of THP-1 cells to macrophages with distinct M(IFNγ+LPS), M(IL-4) and M(IL-10) phenotypes. J Immunol Methods. 2020 Mar

2. Dubin C et al. The IL-4, IL-13 and IL-31 pathways in atopic dermatitis. Expert Rev Clin Immunol. 2021 Aug

Subcellular Location

Secreted.

UNIPROT

SwissProt: P07750 Mouse

Synonyms

B cell growth factor 1 antibody

B cell IgG differentiation factor antibody

B Cell Stimulatory Factor 1 antibody

B-cell stimulatory factor 1 antibody

BCGF 1 antibody

BCGF1 antibody

Binetrakin antibody

BSF-1 antibody

BSF1 antibody

IGG1 induction factor antibody

Expand

Images

  • Sandwich ELISA analysis of Mouse IL-4 matched pair antibodies<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA722262" style="font-weight: bold;text-decoration: underline;">HA722262</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-4 protein (<a href="/products/HA210741" style="font-weight: bold;text-decoration: underline;">HA210741</a>) starting from 2000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA722263" style="font-weight: bold;text-decoration: underline;">HA722263</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

    Sandwich ELISA analysis of Mouse IL-4 matched pair antibodies

    Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722262) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted recombinant standard Mouse IL-4 protein (HA210741) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722263, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.

  • Interpolated concentrations of spiked IL-4 in mouse cell culture media samples.<br />The concentrations of IL-4 were measured in duplicates, interpolated from the IL-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

    Interpolated concentrations of spiked IL-4 in mouse cell culture media samples.
    The concentrations of IL-4 were measured in duplicates, interpolated from the IL-4 standard curves and corrected for sample dilution. Undiluted samples are as follows: cell culture media 50%. The interpolated dilution factor corrected values are plotted (mean +/- SD, n=2).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation