ICOS Recombinant Rabbit Monoclonal Antibody [JE39-99]
Catalog# HA722247
ICOS Recombinant Rabbit Monoclonal Antibody [JE39-99]
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WB
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Mouse
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Rat
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unconjugated
Overview
Product Name
ICOS Recombinant Rabbit Monoclonal Antibody [JE39-99]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human ICOS aa 1-100 / 199.
Species Reactivity
Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 23 kDa
Positive Control
Mouse spleen tissue lysate, mouse thymus tissue lysate, mouse colon tissue lysate, rat spleen tissue lysate, rat thymus tissue lysate.
Conjugation
unconjugated
Clone Number
JE39-99
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
Inducible T-cell costimulator (also called CD278) is an immune checkpoint protein that in humans is encoded by the ICOS (Inducible T-cell COStimulator) gene. The protein belongs to the CD28 and CTLA-4 cell-surface receptor family. These are proteins expressed on the surface of immune cells that mediate signalling between them. A surface protein, the ligand, binds specifically to its receptor on another cell, leading to a signalling cascade in that cell. ICOS is a receptor protein expressed on the surface of activated T cells. Its ligand ICOS-L (previously called B7RP-1) is constitutively expressed on B cells. Stimulation of the ICOS receptor on T cells by ICOS-L on B cells is required for the development of follicular helper T (Tfh) cells. ICOS forms homodimers and plays an important role in cell-cell signaling, immune responses and regulation of cell proliferation.
Background References
1. Peng C et al. Engagement of the costimulatory molecule ICOS in tissues promotes establishment of CD8(+) tissue-resident memory T cells. Immunity. 2022 Jan
2. Zhang X et al. The role of ICOS in allergic disease: Positive or Negative? Int Immunopharmacol. 2022 Feb
Subcellular Location
Cell membrane; Secreted.
Synonyms
Activation inducible lymphocyte immunomediatory molecule antibody
Activation-inducible lymphocyte immunomediatory molecule antibody
AILIM antibody
CD278 antibody
CD278 antigen antibody
CD28-related Protein-1 antibody
CRP1 antibody
CVID1 antibody
ICOS antibody
ICOS_HUMAN antibody
ExpandImages
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Western blot analysis of ICOS on different lysates with Rabbit anti-ICOS antibody (HA722247) at 1/1,000 dilution.
Lane 1: Mouse spleen tissue lysate
Lane 2: Mouse thymus tissue lysate
Lane 3: Mouse colon tissue lysate
Lane 4: Rat spleen tissue lysate
Lane 5: Rat thymus tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 23 kDa
Observed band size: 23 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722247) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"