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CRABP1 Recombinant Rabbit Monoclonal Antibody [JE40-53]

Usd: 385

Specification

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Catalog# HA722241

CRABP1 Recombinant Rabbit Monoclonal Antibody [JE40-53]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

Overview

Product Name

CRABP1 Recombinant Rabbit Monoclonal Antibody [JE40-53]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CRABP1 aa 88-137 / 137.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Tissue

Molecular Weight

Predicted band size: 15 kDa

Positive Control

SH-SY5Y cell lysate, Caco-2 cell lysate, NIH/3T3 cell lysate, mouse liver tissue lysate, e14.0 mouse embryonic brain tissue lysate, human breast cancer tissue, human thyroid tissue, mouse embryo tissue.

Conjugation

unconjugated

Clone Number

JE40-53

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200-1:1,000

  • IF-Tissue

  • 1:50-1:200

Target

Function

Cellular retinoic acid-binding protein 1 is a protein that in humans is encoded by the CRABP1 gene. CRABP1 is assumed to play an important role in retinoic acid-mediated differentiation and proliferation processes. It is structurally similar to the cellular retinol-binding proteins, but binds only retinoic acid. CRABP1 is constitutively expressed and is believed to have different functions in the cell than the related CRABP2.

Background References

1. Nhieu J et al. CRABP1 in Non-Canonical Activities of Retinoic Acid in Health and Diseases. Nutrients. 2022 Apr

2. Lin YL et al. CRABP1-CaMKII-Agrn regulates the maintenance of neuromuscular junction in spinal motor neuron. Cell Death Differ. 2022 Sep

Subcellular Location

Cytoplasm

UNIPROT

SwissProt: P29762 Human

SwissProt: P62965 Mouse

Synonyms

Cellular retinoic acid binding protein 1 antibody

Cellular retinoic acid-binding protein 1 antibody

Cellular retinoic acid-binding protein I antibody

CRABP 1 antibody

CRABP antibody

CRABP I antibody

CRABP-I antibody

Crabp1 antibody

CRABPI antibody

RABP1_HUMAN antibody

Expand

Images

  • Western blot analysis of CRABP1 on different lysates with Rabbit anti-CRABP1 antibody (<a href="/products/HA722241" style="font-weight: bold;text-decoration: underline;">HA722241</a>) at 1/1,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 2: Caco-2 cell lysate (20 µg/Lane)<br />Lane 3: NIH/3T3 cell lysate (20 µg/Lane)<br />Lane 4: Mouse liver tissue lysate (40 µg/Lane)<br />Lane 5: E14.0 mouse embryonic brain tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 15 kDa<br />Observed band size: 15 kDa<br /><br />Exposure time: 14 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722241" style="font-weight: bold;text-decoration: underline;">HA722241</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CRABP1 on different lysates with Rabbit anti-CRABP1 antibody (HA722241) at 1/1,000 dilution.

    Lane 1: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 2: Caco-2 cell lysate (20 µg/Lane)
    Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
    Lane 4: Mouse liver tissue lysate (40 µg/Lane)
    Lane 5: E14.0 mouse embryonic brain tissue lysate (40 µg/Lane)

    Predicted band size: 15 kDa
    Observed band size: 15 kDa

    Exposure time: 14 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722241) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CRABP1 antibody (<a href="/products/HA722241" style="font-weight: bold;text-decoration: underline;">HA722241</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722241" style="font-weight: bold;text-decoration: underline;">HA722241</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human breast cancer tissue with Rabbit anti-CRABP1 antibody (HA722241) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722241) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-CRABP1 antibody (<a href="/products/HA722241" style="font-weight: bold;text-decoration: underline;">HA722241</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722241" style="font-weight: bold;text-decoration: underline;">HA722241</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human thyroid tissue with Rabbit anti-CRABP1 antibody (HA722241) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722241) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-CRABP1 antibody (<a href="/products/HA722241" style="font-weight: bold;text-decoration: underline;">HA722241</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722241" style="font-weight: bold;text-decoration: underline;">HA722241</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse embryo tissue with Rabbit anti-CRABP1 antibody (HA722241) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722241) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue with Rabbit anti-CRABP1 antibody (<a href="/products/HA722241" style="font-weight: bold;text-decoration: underline;">HA722241</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722241" style="font-weight: bold;text-decoration: underline;">HA722241</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse spinal cord tissue with Rabbit anti-CRABP1 antibody (HA722241) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722241) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

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