AHA1 Recombinant Rabbit Monoclonal Antibody [JE35-17]
Catalog# HA722238
AHA1 Recombinant Rabbit Monoclonal Antibody [JE35-17]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
AHA1 Recombinant Rabbit Monoclonal Antibody [JE35-17]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human AHA1 aa 289-338 / 338.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 38 kDa
Positive Control
HeLa cell lysate, A549 cell lysate, NIH/3T3 cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE35-17
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
Target
Function
Activator of 90 kDa heat shock protein ATPase homolog 1 is an enzyme that in humans is encoded by the AHSA1 gene. Acts as a co-chaperone of HSP90AA1. Activates the ATPase activity of HSP90AA1 leading to increase in its chaperone activity. Competes with the inhibitory co-chaperone FNIP1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins. Competes with the inhibitory co-chaperone TSC1 for binding to HSP90AA1, thereby providing a reciprocal regulatory mechanism for chaperoning of client proteins.
Background References
1. Liu X et al. Aha1 Is an Autonomous Chaperone for SULT1A1. Chem Res Toxicol. 2022 Aug
2. Biebl MM et al. p23 and Aha1: Distinct Functions Promote Client Maturation. Subcell Biochem. 2023
Subcellular Location
Cytoplasm, cytosol, Endoplasmic reticulum.
Synonyms
Activator of 90 kDa heat shock protein ATPase homolog 1 antibody
Activator of heat shock 90kDa protein ATPase homolog 1 antibody
AHA 1 antibody
AHA1 antibody
AHSA 1 antibody
Ahsa1 antibody
AHSA1_HUMAN antibody
C14orf3 antibody
HSPC322 antibody
p38 antibody
Images
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Western blot analysis of AHA1 on different lysates with Rabbit anti-AHA1 antibody (HA722238) at 1/1,000 dilution.
Lane 1: HeLa cell lysate (20 µg/Lane)
Lane 2: A549 cell lysate (20 µg/Lane)
Lane 3: NIH/3T3 cell lysate (20 µg/Lane)
Lane 4: C6 cell lysate (20 µg/Lane)
Lane 5: Mouse brain tissue lysate (40 µg/Lane)
Lane 6: Rat brain tissue lysate (40 µg/Lane)
Predicted band size: 38 kDa
Observed band size: 42 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722238) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"