GFAP Recombinant Rabbit Monoclonal Antibody [PSH05-16] - BSA and Azide free (Capture)
Overview
Product Name
GFAP Recombinant Rabbit Monoclonal Antibody [PSH05-16] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human GFAP aa 1-50 / 432.
Species Reactivity
Human, Mouse, Rat
Validated Applications
ELISA(Cap)
Target Molecular Weight
Predicted band size: 50 kDa
Positive Control
Recombinant human GFAP protein (HA210990).
Conjugation
unconjugated
Clone Number
PSH05-16
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Mouse monoclonal [PSH05-17] to GFAP (Detector) (HA601342) and recombinant Human GFAP protein (HA210990) as the standard. The reference range value is 823-200,000 pg/mL.
Target
Function
Glial fibrillary acidic protein (GFAP) is a protein that is encoded by the GFAP gene in humans. It is a type III intermediate filament (IF) protein that is expressed by numerous cell types of the central nervous system (CNS), including astrocytes and ependymal cells during development. GFAP has also been found to be expressed in glomeruli and peritubular fibroblasts taken from rat kidneys,[8] Leydig cells of the testis in both hamsters and humans, human keratinocytes, human osteocytes and chondrocytes and stellate cells of the pancreas and liver in rats. GFAP is closely related to the other three non-epithelial type III IF family members, vimentin, desmin and peripherin, which are all involved in the structure and function of the cell's cytoskeleton. GFAP is thought to help to maintain astrocyte mechanical strength as well as the shape of cells, but its exact function remains poorly understood, despite the number of studies using it as a cell marker.
Background References
1. Abdelhak A et al. Blood GFAP as an emerging biomarker in brain and spinal cord disorders. Nat Rev Neurol. 2022 Mar
2. Kim KY et al. GFAP as a Potential Biomarker for Alzheimer\'s Disease: A Systematic Review and Meta-Analysis. Cells. 2023 May
Subcellular Location
Cytoplasm.
Synonyms
wu:fb34h11 antibody
ALXDRD antibody
cb345 antibody
etID36982.3 antibody
FLJ42474 antibody
FLJ45472 antibody
GFAP antibody
GFAP_HUMAN antibody
gfapl antibody
Glial fibrillary acidic protein antibody
ExpandImages
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Sandwich ELISA analysis of Human GFAP matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722234) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted Recombinant human GFAP protein (HA210990) starting from 100000 pg/ml to 0 pg/ml and detect antibody (HA601342, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
The concentrations of GFAP were interpolated from the GFAP standard curve and corrected for sample dilution. The mean GFAP concentration was determined to be 41.29 ng/ml in rat brain tissue extract. There was no detectable signal in 293T and NIH/3T3 cell extract.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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