NSUN5 Recombinant Rabbit Monoclonal Antibody [PSH05-07]
Catalog# HA722223
NSUN5 Recombinant Rabbit Monoclonal Antibody [PSH05-07]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
NSUN5 Recombinant Rabbit Monoclonal Antibody [PSH05-07]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human NSUN5 aa 51-100 / 429.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 47 kDa
Positive Control
HeLa cell lysate, Caco-2 cell lysate, K-562 cell lysate, A431 cell lysate, MEF cell lysate, PC-12 cell lysate, human kidney tissue lysate, mouse kidney tissue lysate, mouse spleen tissue lysate, rat spleen tissue lysate.
Conjugation
unconjugated
Clone Number
PSH05-07
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
Putative methyltransferase NSUN5 is an enzyme that in humans is encoded by the NSUN5 gene. S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 3782 (m5C3782) in 28S rRNA. m5C3782 promotes protein translation without affecting ribosome biogenesis and fidelity. Required for corpus callosum and cerebral cortex development (By similarity). This gene is deleted in Williams syndrome, a multisystem developmental disorder caused by the deletion of contiguous genes at 7q11.23. Alternative splicing of this gene results in two transcript variants encoding different isoforms.
Background References
1. Liu J et al. The NSUN5-FTH1/FTL pathway mediates ferroptosis in bone marrow-derived mesenchymal stem cells. Cell Death Discov. 2022 Mar
2. Zhang XW et al. NSUN5 promotes progression and predicts poor prognosis in hepatocellular carcinoma. Oncol Lett. 2022 Oct
Subcellular Location
Nucleus, nucleolus.
Synonyms
FLJ10267 antibody
MGC986 antibody
NOL1 antibody
NOL1-related protein antibody
NOL1/NOP2/Sun domain family member 5 antibody
NOL1R antibody
NOP2/Sun domain family, member 5 antibody
NOP2/Sun domain family, member 5A antibody
NSUN5 antibody
NSUN5_HUMAN antibody
ExpandImages
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Western blot analysis of NSUN5 on different lysates with Rabbit anti-NSUN5 antibody (HA722223) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: Caco-2 cell lysate
Lane 3: K-562 cell lysate
Lane 4: A431 cell lysate
Lane 5: MEF cell lysate
Lane 6: PC-12 cell lysate
Lane 7: Human kidney tissue lysate
Lane 8: Mouse kidney tissue lysate
Lane 9: Mouse spleen tissue lysate
Lane 10: Rat spleen tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 47 kDa
Observed band size: 47 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722223) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"