RNase L Recombinant Rabbit Monoclonal Antibody [JE36-11]
Catalog# HA722221
RNase L Recombinant Rabbit Monoclonal Antibody [JE36-11]
-
WB
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Human
-
unconjugated
Overview
Product Name
RNase L Recombinant Rabbit Monoclonal Antibody [JE36-11]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human RNase L aa 492-741 / 741.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 84 kDa
Positive Control
THP-1 cell lysate, THP-1 treated with 20ng/mL IFN-γ for 48 hours cell lysate, 293T cell lysate, HeLa cell lysate.
Conjugation
unconjugated
Clone Number
JE36-11
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Ribonuclease L or RNase L (for latent), known sometimes as ribonuclease 4 or 2'-5' oligoadenylate synthetase-dependent ribonuclease, is an interferon (IFN)-induced ribonuclease which, upon activation, destroys all RNA within the cell (both cellular and viral). RNase L is an enzyme that in humans is encoded by the RNASEL gene. This gene encodes a component of the interferon-regulated 2'-5'oligoadenylate (2'-5'A) system that functions in the antiviral and antiproliferative roles of interferons. RNase L is activated by dimerization, which occurs upon 2'-5'A binding, and results in cleavage of all RNA in the cell. This can lead to activation of MDA5, an RNA helicase involved in the production of interferons.
Background References
1. Boehmer DFR et al. OAS1/RNase L executes RIG-I ligand-dependent tumor cell apoptosis. Sci Immunol. 2021 Jul
2. Burke JM et al. RNase L activation in the cytoplasm induces aberrant processing of mRNAs in the nucleus. PLoS Pathog. 2022 Nov
Subcellular Location
Cytoplasm, Mitochondrion.
UNIPROT
Synonyms
2 5A dependent ribonuclease antibody
2 5A dependent RNase antibody
2' 5' oligoisoadenylate synthetase dependent antibody
2-5A-dependent ribonuclease antibody
2-5A-dependent RNase antibody
DKFZp781D08126 antibody
Interferon induced 2 5A dependent RNase antibody
MGC104972 antibody
MGC133329 antibody
PRCA 1 antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of RNase L on different lysates with Rabbit anti-RNase L antibody (HA722221) at 1/1,000 dilution.
Lane 1: THP-1 cell lysate
Lane 2: THP-1 treated with 20ng/mL IFN-γ for 48 hours cell lysate
Lane 3: 293T cell lysate
Lane 4: HeLa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 84 kDa
Observed band size: 75 kDa
Exposure time: Lane 1-2: 6 seconds; Lane 3-4: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722221) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"