GSK3 alpha Recombinant Rabbit Monoclonal Antibody [JE33-27] (HA722217)
Catalog# HA722217
Rabbit Monoclonal to GSK3 alpha
Tested for
-
WB
-
IHC-P
Reacts with
-
Human
-
Mouse
-
Rat
-
Monkey
Recombinant production enables lot-to-lot consistency and is animal-cruelty-free
Western blot analysis of GSK3 alpha on different lysates with Rabbit anti-GSK3 alpha antibody (HA722217) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HCT 116cell lysate
Lane 6: HEK-293 cell lysate
Lane 7: U-87 MG cell lysate
Lane 8: MCF7 cell lysate
Lane 9: MDA-MB-231 cell lysate
Lane 10: SK-Br-3 cell lysate
Lane 11: Neuro-2a cell lysate
Lane 12: NIH/3T3 cell lysate
Lane 13: C6 cell lysate
Lane 14: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722217) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Lane 1: HeLa cell lysate
Lane 2: A431 cell lysate
Lane 3: A549 cell lysate
Lane 4: Jurkat cell lysate
Lane 5: HCT 116cell lysate
Lane 6: HEK-293 cell lysate
Lane 7: U-87 MG cell lysate
Lane 8: MCF7 cell lysate
Lane 9: MDA-MB-231 cell lysate
Lane 10: SK-Br-3 cell lysate
Lane 11: Neuro-2a cell lysate
Lane 12: NIH/3T3 cell lysate
Lane 13: C6 cell lysate
Lane 14: COS-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 51 kDa
Observed band size: 51 kDa
Exposure time: 42 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722217) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.