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TFIIB Recombinant Rabbit Monoclonal Antibody [JE38-37]

Usd: 385

Specification

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Catalog# HA722213

TFIIB Recombinant Rabbit Monoclonal Antibody [JE38-37]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

TFIIB Recombinant Rabbit Monoclonal Antibody [JE38-37]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human TFIIB aa 217-316 / 316.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 35 kDa

Positive Control

HepG2 cell lysate, HeLa cell lysate, Jurkat cell lysate, K-562 cell lysate, C6 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate.

Conjugation

unconjugated

Clone Number

JE38-37

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Transcription factor II B (TFIIB) is a general transcription factor that is involved in the formation of the RNA polymerase II preinitiation complex (PIC) and aids in stimulating transcription initiation. TFIIB is localised to the nucleus and provides a platform for PIC formation by binding and stabilising the DNA-TBP (TATA-binding protein) complex and by recruiting RNA polymerase II and other transcription factors. It is encoded by the TFIIB gene, and is homologous to archaeal transcription factor B and analogous to bacterial sigma factors.

Background References

1. O\'Brien MJ et al. Beyond the canonical role of TFIIB in eukaryotic transcription. Curr Genet. 2022 Feb

2. O\'Brien MJ et al. Critical Involvement of TFIIB in Viral Pathogenesis. Front Mol Biosci. 2021 Apr

Subcellular Location

Nucleus, Chromosome.

UNIPROT

SwissProt: Q00403 Human

SwissProt: P62915 Mouse

SwissProt: P62916 Rat

Synonyms

General transcription factor IIB antibody

General transcription factor TFIIB antibody

gtf2b antibody

RNA polymerase II transcription factor IIB antibody

S300 II antibody

S300-II antibody

TF IIB antibody

TF2B antibody

TF2B_HUMAN antibody

TFIIB antibody

Expand

Images

  • Western blot analysis of TFIIB on different lysates with Rabbit anti-TFIIB antibody (<a href="/products/HA722213" style="font-weight: bold;text-decoration: underline;">HA722213</a>) at 1/1,000 dilution.<br /><br />Lane 1: HepG2 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: K-562 cell lysate<br />Lane 5: C6 cell lysate<br />Lane 6: RAW264.7 cell lysate<br />Lane 7: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 35 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 3 minutes; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722213" style="font-weight: bold;text-decoration: underline;">HA722213</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TFIIB on different lysates with Rabbit anti-TFIIB antibody (HA722213) at 1/1,000 dilution.

    Lane 1: HepG2 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: K-562 cell lysate
    Lane 5: C6 cell lysate
    Lane 6: RAW264.7 cell lysate
    Lane 7: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 35 kDa
    Observed band size: 30 kDa

    Exposure time: 3 minutes; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722213) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"