EIF2B5 Recombinant Rabbit Monoclonal Antibody [JE34-98]
Catalog# HA722206
EIF2B5 Recombinant Rabbit Monoclonal Antibody [JE34-98]
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WB
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IF-Cell
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Human
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unconjugated
Overview
Product Name
EIF2B5 Recombinant Rabbit Monoclonal Antibody [JE34-98]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human EIF2B5 aa 1-50 / 721.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 80 kDa
Positive Control
HeLa cell lysate, 293T cell lysate, Jurkat cell lysate, HeLa.
Conjugation
unconjugated
Clone Number
JE34-98
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
Target
Function
Acts as a component of the translation initiation factor 2B (eIF2B) complex, which catalyzes the exchange of GDP for GTP on eukaryotic initiation factor 2 (eIF2) gamma subunit. Its guanine nucleotide exchange factor activity is repressed when bound to eIF2 complex phosphorylated on the alpha subunit, thereby limiting the amount of methionyl-initiator methionine tRNA available to the ribosome and consequently global translation is repressed. Activated by the chemical integrated stress response (ISR) inhibitor ISRIB which stimulates guanine nucleotide exchange factor activity for both phosphorylated and unphosphorylated eIF2.
Background References
1. He SL et al. CircAHNAK upregulates EIF2B5 expression to inhibit the progression of ovarian cancer by modulating the JAK2/STAT3 signaling pathway. Carcinogenesis. 2022 Nov
2. He SL et al. LncRNA KCNQ1OT1 promotes the metastasis of ovarian cancer by increasing the methylation of EIF2B5 promoter. Mol Med. 2022 Sep
Subcellular Location
Cytoplasm, cytosol.
UNIPROT
Synonyms
CACH antibody
CLE antibody
EI2BE_HUMAN antibody
EIF 2B antibody
eIF 2B GDP GTP exchange factor antibody
eIF 2B GDP GTP exchange factor subunit epsilon antibody
eIF-2B GDP-GTP exchange factor subunit epsilon antibody
EIF2B antibody
EIF2B5 antibody
EIF2BE antibody
ExpandImages
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Western blot analysis of EIF2B5 on different lysates with Rabbit anti-EIF2B5 antibody (HA722206) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: 293T cell lysate
Lane 3: Jurkat cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 80 kDa
Observed band size: 80 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722206) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HeLa cells labeling EIF2B5 with Rabbit anti-EIF2B5 antibody (HA722206) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EIF2B5 antibody (HA722206) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"