KIFC1 Recombinant Rabbit Monoclonal Antibody [JE00-46] (HA722197)
Catalog# HA722197
Rabbit Monoclonal to KIFC1
-
WB
-
IF-Cell
-
IHC-P
-
FC
-
Human
Lane 1: Jurkat cell lysate
Lane 2: HT-29 cell lysate
Lane 3: HeLa cell lysate
Lane 4: MCF7 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 74 kDa
Observed band size: 70 kDa
Exposure time: 43 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722197) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
KIFC1 Recombinant Rabbit Monoclonal Antibody [JE00-46] (HA722197)
Immunogen
Synthetic peptide within human kifc1 aa 1-100.
Host
Rabbit
Positive Control
Jurkat cell lysate, HT-29 cell lysate, HeLa cell lysate, MCF7 cell lysate, Jurkat, human testis tissue, human stomach tissue, human stomach cancer tissue.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
JE00-46
APPLICATION DILUTION
-
WB
-
1:1,000
-
IF-Cell
-
1:100
-
IHC-P
-
1:200-1:1,000
-
FC
-
1:1,000
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 74 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
KIFC1
SYNONYMS
HSET antibody;HSET KNSL2 antibody;KIF C1 antibody;KIFC 1 antibody;KIFC1 antibody;KIFC1_HUMAN antibody;Kinesin family member C1 antibody;Kinesin like 2 antibody;Kinesin like protein 2 antibody;Kinesin like protein KIFC1 antibody;Kinesin related protein HSET antibody;Kinesin-like protein 2 antibody;Kinesin-like protein KIFC1 antibody;Kinesin-related protein HSET antibody;KNSL 2 antibody;KNSL2 antibody;MGC1202 antibody;MGC149736 antibody;MGC149737 antibody;Mitotic kinesin like protein 2 antibody;MKLP 2 antibody;MKLP2 antibody;RAB6 KIFL antibody;Rabkinesin 6 antibody
SUBCELLULAR LOCATION
Nucleus, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome, spindle, Early endosome.
FUNCTION
Kinesin-like protein KIFC1 is a protein that in humans is encoded by the KIFC1 gene. The protein KifC1 is a member of kinesin-14 family. KifC1 consists of C-terminal motor domain, superhelical stalk and N-terminal tail domain. Tail and motor domains contain microtubule-binding sites. This kinesin moves towards the minus-end of microtubule and has an ability to slide or crosslink microtubules. KifC1 functions during mitotic spindle formation.
CITATIONS
-
Transport of Acrosomal Enzymes by KIFC1 via the Acroframosomal Cytoskeleton during Spermatogenesis in Macrobrachium rosenbergii (Crustacea, Decapoda, Malacostracea)
-
ICC
-
Human
-
Citation
-
