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TPH2 Recombinant Rabbit Monoclonal Antibody [PSH04-89]

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Specification

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Catalog# HA722195

TPH2 Recombinant Rabbit Monoclonal Antibody [PSH04-89]

Application

  • WB

  • IHC-P

  • IHC-Fr

  • IP

Reactivity

  • Mouse

  • Rat

BSA and Azide free
Predicted reactivity

  • Cynomolgus monkey

  • Pig

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

TPH2 Recombinant Rabbit Monoclonal Antibody [PSH04-89]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein with Human THP2 aa 1-490.

Species Reactivity

Mouse, Rat (Predicted: Cynomolgus monkey, Pig)

Predicted species support after-sales service

Validated Applications

WB, IHC-P, IHC-Fr, IP

Target Molecular Weight

Predicted band size: 56 kDa

Positive Control

Mouse hippocampus tissue lysate, mouse brain tissue lysate, rat brain tissue lysate, rat hippocampus tissue lysate, mouse midbrain tissue, mouse cerebrum tissue, rat cerebrum tissue, mouse brain (raphe nucleus) tissue.

Conjugation

unconjugated

Clone Number

PSH04-89

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:2,000

  • IHC-P

  • 1:500

  • IHC-Fr

  • 1:500

  • IP

  • 1-2μg/sample

Target

Function

Tryptophan hydroxylase 2 (TPH2) is an isozyme of tryptophan hydroxylase found in vertebrates. In humans, TPH2 is primarily expressed in the serotonergic neurons of the brain, with the highest expression in the raphe nucleus of the midbrain. Until the discovery of TPH2 in 2003, serotonin levels in the central nervous system were believed to be regulated by serotonin synthesis in peripheral tissues, in which tryptophan hydroxylase is the dominant form.

Background References

1. Zaniewska M et al. Tph2 Gene Expression Defines Ethanol Drinking Behavior in Mice. Cells. 2022 Mar

2. Liu H et al. TPH2 in the Dorsal Raphe Nuclei Regulates Energy Balance in a Sex-Dependent Manner. Endocrinology. 2021 Jan

Subcellular Location

Cytosol, neuron projection.

UNIPROT

SwissProt: Q8CGV2 Mouse

SwissProt: Q8CGU9 Rat

Synonyms

MGC138871 antibody

ADHD7 antibody

FLJ37295 antibody

MGC138872 antibody

Neuronal tryptophan hydroxylase antibody

NTPH antibody

TPH 2 antibody

Tph2 antibody

TPH2_HUMAN antibody

Tryptophan 5-hydroxylase 2 antibody

Expand

Images

  • Application: IHC-Fr<br /><br />Species: Mouse<br /><br />Site: Raphe nucleus<br /><br />Sample: Frozen section<br /><br />Antibody concentration: 1/500<br /><br />Antigen retrieval: Not required

    Application: IHC-Fr

    Species: Mouse

    Site: Raphe nucleus

    Sample: Frozen section

    Antibody concentration: 1/500

    Antigen retrieval: Not required

  • Immunohistochemical analysis of paraffin-embedded mouse midbrain tissue with Rabbit anti-TPH2 antibody (<a href="/products/HA722195" style="font-weight: bold;text-decoration: underline;">HA722195</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722195" style="font-weight: bold;text-decoration: underline;">HA722195</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse midbrain tissue with Rabbit anti-TPH2 antibody (HA722195) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722195) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-TPH2 antibody (<a href="/products/HA722195" style="font-weight: bold;text-decoration: underline;">HA722195</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722195" style="font-weight: bold;text-decoration: underline;">HA722195</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse cerebrum tissue with Rabbit anti-TPH2 antibody (HA722195) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722195) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-TPH2 antibody (<a href="/products/HA722195" style="font-weight: bold;text-decoration: underline;">HA722195</a>) at 1/500 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722195" style="font-weight: bold;text-decoration: underline;">HA722195</a>) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded rat cerebrum tissue with Rabbit anti-TPH2 antibody (HA722195) at 1/500 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722195) at 1/500 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Relative expression (RE)</span><br /><br />Western blot analysis of TPH2 on different lysates with Rabbit anti-TPH2 antibody (<a href="/products/HA722195" style="font-weight: bold;text-decoration: underline;">HA722195</a>) at 1/2,000 dilution.<br /><br />Lane 1: Mouse brain tissue lysate<br />Lane 2: Mouse kidney tissue lysate (negative)<br />Lane 3: Rat brain tissue lysate<br />Lane 4: Rat kidney tissue lysate (negative)<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 56 kDa<br />Observed band size: 50 kDa<br /><br />Exposure time: 1 minute 2 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722195" style="font-weight: bold;text-decoration: underline;">HA722195</a>) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Relative expression (RE)

    Western blot analysis of TPH2 on different lysates with Rabbit anti-TPH2 antibody (HA722195) at 1/2,000 dilution.

    Lane 1: Mouse brain tissue lysate
    Lane 2: Mouse kidney tissue lysate (negative)
    Lane 3: Rat brain tissue lysate
    Lane 4: Rat kidney tissue lysate (negative)

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 56 kDa
    Observed band size: 50 kDa

    Exposure time: 1 minute 2 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722195) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • TPH2 was immunoprecipitated from 0.2 mg rat brain tissue lysate with <a href="/products/HA722195" style="font-weight: bold;text-decoration: underline;">HA722195</a> at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using <a href="/products/HA722195" style="font-weight: bold;text-decoration: underline;">HA722195</a> at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.<br /><br />Lane 1: rat brain tissue lysate (input)<br />Lane 2: <a href="/products/HA722195" style="font-weight: bold;text-decoration: underline;">HA722195</a> IP in rat brain tissue lysate<br />Lane 3: Rabbit IgG instead of <a href="/products/HA722195" style="font-weight: bold;text-decoration: underline;">HA722195</a> in rat brain tissue lysate<br /><br />Blocking/Dilution buffer: 5% NFDM/TBST<br />Exposure time: 3 minutes; ECL: <a href="/products/K1801" style="font-weight: bold;text-decoration: underline;">K1801</a>

    TPH2 was immunoprecipitated from 0.2 mg rat brain tissue lysate with HA722195 at 2 µg/10 µl beads. Western blot was performed from the immunoprecipitate using HA722195 at 1/1,000 dilution. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.

    Lane 1: rat brain tissue lysate (input)
    Lane 2: HA722195 IP in rat brain tissue lysate
    Lane 3: Rabbit IgG instead of HA722195 in rat brain tissue lysate

    Blocking/Dilution buffer: 5% NFDM/TBST
    Exposure time: 3 minutes; ECL: K1801

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation