Phospho-ErbB3/HER3 (Y1289) Recombinant Rabbit Monoclonal Antibody [JE51-25]
Catalog# HA722178
Phospho-ErbB3/HER3 (Y1289) Recombinant Rabbit Monoclonal Antibody [JE51-25]
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WB
-
IF-Cell
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Human
-
unconjugated
Overview
Product Name
Phospho-ErbB3/HER3 (Y1289) Recombinant Rabbit Monoclonal Antibody [JE51-25]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phosphopeptide corresponding to a region surrounding Tyr1289 of Human ErbB3.
Species Reactivity
Human
Validated Applications
WB, IF-Cell
Molecular Weight
Predicted band size: 148 kDa
Positive Control
MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, MCF7 cells serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes.
Conjugation
unconjugated
Clone Number
JE51-25
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IF-Cell
-
1:100
Target
Function
The EGF receptor family comprises several related receptor tyrosine kinases that are frequently overexpressed in a variety of carcinomas. Members of this receptor family include EGFR (HER1), Neu (ErbB-2, HER2), ErbB-3 (HER3) and ErbB-4 (HER4), which form either homodimers or heterodimers upon ligand binding. Full length ErbB-3 is overexpressed in human mammary tumors. The ErbB-3 gene also produces several alternative variants, including a secreted form which negatively regulates heregulin stimulated ErbB activation. ErbB-3 heterodimerizes with Neu and binds heregulin in order to activate phosphoinositide (PI) 3-kinase. The recruitment and activation of PI 3-kinase occurs via its interaction with phosphorylated YXXM motifs in the carboxy terminus of ErbB-3.
Background References
1. Sağsöz H. et. al. Expression and localisation of epidermal growth factor receptors and their ligands in the lower genital tract of cycling cows. Reprod Fertil Dev. 2019 Jul 4.
2. Dietrich M. et. al. Protein kinase C regulates ErbB3 turnover. Exp Cell Res. 2019 Jun 21.
Subcellular Location
Cell membrane, secreted.
UNIPROT
Synonyms
c erbB 3 antibody
c erbB3 antibody
Erb b2 receptor tyrosine kinase 3 antibody
ErbB 3 antibody
ERBB3 antibody
ERBB3 protein antibody
erbB3 S antibody
ERBB3_HUMAN antibody
Glial growth factor receptor antibody
HER 3 antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-ErbB3/HER3 (Y1289) on different lysates with Rabbit anti-Phospho-ErbB3/HER3 (Y1289) antibody (HA722178) at 1/1,000 dilution.
Lane 1: MCF7 cell lysate
Lane 2: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate
Lane 3: MCF7 serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 20 µg/Lane.
Predicted band size: 148 kDa
Observed band size: 180 kDa
Exposure time: 3 minutes; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722178) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of MCF7 cells serum starved for 6 hours then add 10nM Neuregulin-1 for 10 minutes labeling Phospho-ErbB3/HER3 (Y1289) with Rabbit anti-Phospho-ErbB3/HER3 (Y1289) antibody (HA722178) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Phospho-ErbB3/HER3 (Y1289) antibody (HA722178) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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