Mouse IFN beta Recombinant Rabbit Monoclonal Antibody [PSH04-73] - BSA and Azide free (Detector)
Overview
Product Name
Mouse IFN beta Recombinant Rabbit Monoclonal Antibody [PSH04-73] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Mouse IFN beta aa 22-182 (P01575).
Species Reactivity
Mouse
Validated Applications
ELISA(Det)
Positive Control
Recombinant Mouse IFN beta protein (HA210789).
Conjugation
unconjugated
Clone Number
PSH04-73
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-72] to Mouse IFN beta antibody (Capture) (HA722172) and Recombinant Mouse IFN beta protein (HA210789) as the standard. The reference range value is 62.5-2,000 pg/mL.
Target
Function
This gene encodes a cytokine that belongs to the interferon family of signaling proteins, which are released as part of the innate immune response to pathogens. The protein encoded by this gene belongs to the type I class of interferons, which are important for defense against viral infections. In addition, type I interferons are involved in cell differentiation and anti-tumor defenses. Following secretion in response to a pathogen, type I interferons bind a homologous receptor complex and induce transcription of genes such as those encoding inflammatory cytokines and chemokines. Overactivation of type I interferon secretion is linked to autoimmune diseases. Mice deficient for this gene display several phenotypes including defects in B cell maturation and increased susceptibility to viral infection.
Background References
1. Tresse E et al. IFN-β rescues neurodegeneration by regulating mitochondrial fission via STAT5, PGAM5, and Drp1. EMBO J. 2021 Jun
2. Ko R et al. Pim1 promotes IFN-β production by interacting with IRF3. Exp Mol Med. 2022 Nov
Subcellular Location
Secreted.
UNIPROT
Synonyms
beta-interferon antibody
Fibroblast interferon antibody
IFB antibody
IFF antibody
IFN beta antibody
IFN-beta antibody
IFNB 1 antibody
IFNB antibody
IFNB_HUMAN antibody
IFNB1 antibody
ExpandImages
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![Sandwich ELISA analysis of Mouse IFN beta matched pair antibodies<br /><br />Capture: <a href="/products/HA722172" style="font-weight: bold;text-decoration: underline;">HA722172</a>, Mouse IFN beta Rabbit mAb [PSH04-72]<br />Detector: <a href="/products/HA722173" style="font-weight: bold;text-decoration: underline;">HA722173</a>, Mouse IFN beta Rabbit mAb [PSH04-73]<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA722172" style="font-weight: bold;text-decoration: underline;">HA722172</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Recombinant Mouse IFN beta protein (<a href="/products/HA210789" style="font-weight: bold;text-decoration: underline;">HA210789</a>) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (<a href="/products/HA722173" style="font-weight: bold;text-decoration: underline;">HA722173</a>, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA722173_1.jpg?v=20260908003346)
Sandwich ELISA analysis of Mouse IFN beta matched pair antibodies
Capture: HA722172, Mouse IFN beta Rabbit mAb [PSH04-72]
Detector: HA722173, Mouse IFN beta Rabbit mAb [PSH04-73]
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722172) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Recombinant Mouse IFN beta protein (HA210789) starting from 2,000 pg/ml to 0 pg/ml and detect antibody (HA722173, Biotin, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"