Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) Recombinant Rabbit Monoclonal Antibody [JE57-42]
Catalog# HA722146
Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) Recombinant Rabbit Monoclonal Antibody [JE57-42]
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WB
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Human
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unconjugated
Overview
Product Name
Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) Recombinant Rabbit Monoclonal Antibody [JE57-42]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Ser474 of human PAK4.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 64/81/75 kDa
Positive Control
A549 cell lysate, HeLa cell lysate, MCF7 cell lysate, HUVEC cell lysate, HUVEC treated with 50ng/mL VEGF for 2 minutes cell lysate.
Conjugation
unconjugated
Clone Number
JE57-42
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Serine/threonine-protein kinase PAK 4 is an enzyme that in humans is encoded by the PAK4 gene. Serine/threonine-protein kinase PAK 5 is an enzyme that in humans is encoded by the PAK5 gene. Serine/threonine-protein kinase PAK 6 is an enzyme that in humans is encoded by the PAK6 gene. PAK4 is one of six members of the PAK family of serine/threonine kinases which are divided into group I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK6 and PAK5/7). PAK4 localizes in sub-cellular domains of the cytoplasm and nucleus. PAK4 regulates cytoskeleton remodeling, phenotypic signaling and gene expression, and affects directional motility, invasion, metastasis, and growth. Similar to PAK1, PAK4-signaling-dependent cellular functions also regulate both physiologic and disease processes such as cancer, as PAK4 is overexpressed and/or hyperstimulated in human cancer, at-large. The PAK5 enzyme is one of three members of Group II PAK family of serine/threonine kinases, and evolutionary conserved across species. PAK6 belongs to the Group II PAK family members. Like other family members, PAK6 is also evolutionary conserved across species. Phospho-PAK4+PAK5+PAK6 (S474+S560+S602) Antibody detects endogenous levels of PAK4, PAK5 and PAK6 only when phosphorylated at serine 474, 602, or 560, respectively.
Background References
1. Minden A. PAK4-6 in cancer and neuronal development. Cell Logist. 2012 Apr
Subcellular Location
Cytoplasm; Mitochondrion, Cytoplasm, Nucleus; Cytoplasm, Nucleus.
Synonyms
KIAA1142 antibody
p21 activated kinase 4 antibody
p21 Cdc42/Rac1-actiated kinase 4 antibody
P21 protein (Cdc42/Rac) activated kinase 4 antibody
p21(CDKN1A) activated kinase 4 antibody
p21-activated kinase 4 antibody
PAK 4 antibody
PAK-4 antibody
Pak4 antibody
PAK4_HUMAN antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) on different lysates with Rabbit anti-Phospho-PAK4+PAK5+PAK6 (S474 + S560 + S602) antibody (HA722146) at 1/1,000 dilution.
Lane 1: A549 cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: MCF7 cell lysate (20 µg/Lane)
Lane 4: HUVEC cell lysate (10 µg/Lane)
Lane 5: HUVEC treated with 50ng/mL VEGF for 2 minutes cell lysate (30 µg/Lane)
Predicted band size: 64/81/75 kDa
Observed band size: 64 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722146) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"