Phospho-Nucleophosmin (T199) Recombinant Rabbit Monoclonal Antibody [JE63-16]
Catalog# HA722125
Phospho-Nucleophosmin (T199) Recombinant Rabbit Monoclonal Antibody [JE63-16]
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WB
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Human
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unconjugated
Overview
Product Name
Phospho-Nucleophosmin (T199) Recombinant Rabbit Monoclonal Antibody [JE63-16]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic phosphopeptide corresponding to residues surrounding Thr199 of Human Nucleophosmin.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 33 kDa
Positive Control
HeLa cell lysate, HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate.
Conjugation
unconjugated
Clone Number
JE63-16
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000-1:5,000
Target
Function
Nucleophosmin (NPM), also known as nucleolar phosphoprotein B23 or numatrin, is a protein that in humans is encoded by the NPM1 gene. NPM1 is associated with nucleolar ribonucleoprotein structures and binds single-stranded and double-stranded nucleic acids, but it binds preferentially G-quadruplex forming nucleic acids. It is involved in the biogenesis of ribosomes and may assist small basic proteins in their transport to the nucleolus. Its regulation through SUMOylation (by SENP3 and SENP5) is another facet of the protein's regulation and cellular functions. It is located in the nucleolus, but it can be translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs.
Background References
1. Cela I et al. Nucleophosmin in Its Interaction with Ligands. Int J Mol Sci. 2020 Jul
2. Sekhar KR et al. Nucleophosmin Plays a Role in Repairing DNA Damage and Is a Target for Cancer Treatment. Cancer Res. 2023 May
Subcellular Location
Nucleus, nucleolus, nucleoplasm, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome
UNIPROT
Synonyms
B23 antibody
MGC104254 antibody
NO38 antibody
NPM antibody
NPM_HUMAN antibody
NPM1 antibody
Nucleolar phosphoprotein B23 antibody
Nucleolar protein NO38 antibody
Nucleophosmin (nucleolar phosphoprotein B23 numatrin) antibody
Nucleophosmin antibody
ExpandImages
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☑ Cell treatment (CT)
Western blot analysis of Phospho-Nucleophosmin (T199) on different lysates with Rabbit anti-Phospho-Nucleophosmin (T199) antibody (HA722125) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate
Lane 3: HeLa treated with 100ng/mL Nocodazole for 17 hours cell lysate, then the membrane treated with λpp for 1 hour
Lysates/proteins at 15 µg/Lane.
Predicted band size: 33 kDa
Observed band size: 38 kDa
Exposure time: 2 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722125) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"