EIF2S1 Recombinant Rabbit Monoclonal Antibody [PSH04-29] (HA722112)
Catalog# HA722112
Rabbit Monoclonal to EIF2S1
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WB
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IF-Cell
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IHC-P
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FC
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IP
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Human
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Mouse
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Rat
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Monkey
Lane 1: MCF7 cell lysate
Lane 2: HepG2 cell lysate
Lane 3: HeLa cell lysate
Lane 4: COS-1 cell lysate
Lane 5: A549 cell lysate
Lane 6: RAW264.7 cell lysate
Lane 7: C6 cell lysate
Lane 8: Mouse kidney tissue lysate
Lane 9: Mouse spleen tissue lysate
Lane 10: Rat kidney tissue lysate
Lane 11: Rat spleen tissue lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 36 kDa
Observed band size: 36 kDa
Exposure time: 59 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722112) at 1/2,000 dilution and competitor's antibody at 1/2,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Specifications
Product Type
Recombinant Rabbit monoclonal primary
Product Name
EIF2S1 Recombinant Rabbit Monoclonal Antibody [PSH04-29] (HA722112)
Immunogen
Recombinant protein within human eif2s1 aa 1-315 / 315.
Host
Rabbit
Positive Control
MCF7 cell lysate, HepG2 cell lysate, HeLa cell lysate, COS-1 cell lysate, A549 cell lysate, RAW264.7 cell lysate, C6 cell lysate, mouse kidney tissue lysate, mouse spleen tissue lysate, rat kidney tissue lysate, rat spleen tissue lysate, human breast tissue, mouse breast tissue, rat breast tissue, HepG2.
Conjugation
Unconjugated
Clonality
Monoclonal
Clone Number
PSH04-29
APPLICATION DILUTION
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WB
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1:2,000
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IF-Cell
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1:100
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IHC-P
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1:1,000
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FC
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1:1,000
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IP
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1-2μg/sample
PROPERTIES
Form
Liquid
Storage Condition
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). It is recommended to aliquot into single-use upon delivery. Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Concentration
1ug/ul
Purification
Protein A affinity purified.
Molecular Weight
Predicted band size: 36 kDa
Isotype
IgG
TARGET
UNIPROT #
PROTEIN NAME
EIF2S1
SYNONYMS
EIF 2 alpha antibody;EIF 2 antibody;EIF 2A antibody;EIF 2alpha antibody;eIF-2-alpha antibody;eIF-2A antibody;EIF-2alpha antibody;EIF2 alpha antibody;EIF2 antibody;EIF2A antibody;EIF2S1 antibody;Eukaryotic translation initiation factor 2 subunit 1 alpha 35kDa antibody;Eukaryotic translation initiation factor 2 subunit 1 alpha antibody;Eukaryotic translation initiation factor 2 subunit 1 antibody;Eukaryotic translation initiation factor 2 subunit alpha antibody;IF2A_HUMAN antibody
SUBCELLULAR LOCATION
Cytoplasm, Stress granule.
FUNCTION
Eukaryotic translation initiation factor 2 subunit 1 (eIF2α) is a protein that in humans is encoded by the EIF2S1 gene. The protein encoded by this gene is the alpha (α) subunit of the translation initiation factor eIF2 protein complex which catalyzes an early regulated step of protein synthesis initiation, promoting the binding of the initiator tRNA (Met-tRNAiMet) to 40S ribosomal subunits. Binding occurs as a ternary complex of methionyl-tRNA, eIF2, and GTP. eIF2 is composed of 3 nonidentical subunits, alpha (α, 36 kD, this article), beta (β, 38 kD), and gamma (γ, 52 kD). The rate of formation of the ternary complex is modulated by the phosphorylation state of eIF2α. Phosphorylation of eIF2α by EIF-2 kinases plays a key role in regulating the integrated stress response.
CITATIONS
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Qu Jiayuan,et al
Polystyrene microplastics trigger testosterone decline via GPX1
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WB
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Mouse
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Citation
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