Human CRP Recombinant Rabbit Monoclonal Antibody [PSH04-26] - BSA and Azide free (Detector)
Overview
Product Name
Human CRP Recombinant Rabbit Monoclonal Antibody [PSH04-26] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human CRP aa 19-224 (P02741).
Species Reactivity
Human, Dog, Cynomolgus monkey
Validated Applications
ELISA(Det)
Target Molecular Weight
Predicted band size: 25.0 kDa
Positive Control
Recombinant Human CRP protein (HA210648).
Conjugation
unconjugated
Clone Number
PSH04-26
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-25] to Human CRP (Capture) (HA722108) and recombinant standard Human CRP protein (HA210648). The reference range value is 617-50,000 pg/mL.
Target
Function
C-reactive protein (CRP) is an annular (ring-shaped) pentameric protein found in blood plasma, whose circulating concentrations rise in response to inflammation. It is an acute-phase protein of hepatic origin that increases following interleukin-6 secretion by macrophages and T cells. Its physiological role is to bind to lysophosphatidylcholine expressed on the surface of dead or dying cells (and some types of bacteria) in order to activate the complement system via C1q. CRP is synthesized by the liver in response to factors released by macrophages and fat cells (adipocytes). It is a member of the pentraxin family of proteins. It is not related to C-peptide (insulin) or protein C (blood coagulation). C-reactive protein was the first pattern recognition receptor (PRR) to be identified.
Background References
1. Zhu M et al. C-reactive protein and cancer risk: a pan-cancer study of prospective cohort and Mendelian randomization analysis. BMC Med. 2022 Sep
2. Pathak A et al. Evolution of C-Reactive Protein. Front Immunol. 2019 Apr
Subcellular Location
Secreted.
UNIPROT
Synonyms
Pentraxin 1, short antibody
C reactive protein antibody
C reactive protein pentraxin related antibody
C-reactive protein(1-205) antibody
CRP antibody
CRP_HUMAN antibody
MGC88244 antibody
Pentraxin 1 antibody
PTX 1 antibody
PTX1 antibody
Images
-
Sandwich ELISA analysis of Human CRP matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722108) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human CRP protein (HA210648) starting from 50,000 pg/ml to 0 pg/ml and detect antibody (HA722109) (Biotin-conjugated, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
The concentrations of CRP were interpolated from the CRP standard curves and corrected for sample dilution.Undiluted sample is serum 1: 200,000. The mean CRP concentration was determined to be 85,871 ng/ml in mixed serum from twenty-four volunteers.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Human CRP Recombinant Rabbit Monoclonal Antibody [PSH04-25] - BSA and Azide free (Capture)
Application: ELISA(Cap)
Reactivity: Human,Dog,Cynomolgus monkey
Conjugate: unconjugated
