Human IL-15 Recombinant Rabbit Monoclonal Antibody [PSH04-19] - BSA and Azide free (Capture)
Overview
Product Name
Human IL-15 Recombinant Rabbit Monoclonal Antibody [PSH04-19] - BSA and Azide free (Capture)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human IL-15 aa 49-162 (P40933).
Species Reactivity
Human
Validated Applications
ELISA(Cap)
Target Molecular Weight
Predicted band size: 18.1 kDa
Positive Control
Recombinant standard Human IL-15 protein (HA210773).
Conjugation
unconjugated
Clone Number
PSH04-19
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Cap)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH04-20] to Human IL-15 (Detector) (HA722103) and recombinant standard Human IL-15 protein (HA210773). The reference range value is 12.3-1,000 pg/mL.
Target
Function
Interleukin-15 (IL-15) is a protein that in humans is encoded by the IL15 gene. IL-15 is an inflammatory cytokine with structural similarity to Interleukin-2 (IL-2). Like IL-2, IL-15 binds to and signals through a complex composed of IL-2/IL-15 receptor beta chain (CD122) and the common gamma chain (gamma-C, CD132). IL-15 is secreted by mononuclear phagocytes (and some other cells) following infection by virus(es). This cytokine induces the proliferation of natural killer cells, i.e. cells of the innate immune system whose principal role is to kill virally infected cells.
Background References
1. Zannikou M et al. IL15 modification enables CAR T cells to act as a dual targeting agent against tumor cells and myeloid-derived suppressor cells in GBM. J Immunother Cancer. 2023 Feb
2. Xu Y et al. An Engineered IL15 Cytokine Mutein Fused to an Anti-PD1 Improves Intratumoral T-cell Function and Antitumor Immunity. Cancer Immunol Res. 2021 Oct
Subcellular Location
Secreted; Nucleus.
UNIPROT
Synonyms
IL 15 antibody
IL-15 antibody
IL15 antibody
IL15_HUMAN antibody
Interleukin 15 antibody
Interleukin-15 antibody
Interleukin15 antibody
MGC9721 antibody
Images
-
Sandwich ELISA analysis of Human IL-15 matched pair antibodies
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA722102) diluted in carbonate/bicarbonate buffer, at a concentration of 5 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1% BSA blocking buffer, and incubated with serial diluted Recombinant Human IL-15 protein (HA210773) starting from 2000 pg/ml to 0 pg/ml and detect antibody (HA722103) (Biotin-conjugated, 0.2 µg/ml) for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm. -
The concentrations of IL-15 were interpolated from the IL-15 standard curves. The mean IL-15 concentration was determined to be 65 pg/mL in HuT-102 supernatant. There was no detectable signal in THP-1 supernatant and human serum.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Biotin Conjugated Human IL-15 Recombinant Rabbit Monoclonal Antibody [PSH04-20] - Detector
Application: ELISA(Det)
Reactivity: Human
Conjugate: Biotin
