FBXO3 Recombinant Rabbit Monoclonal Antibody [PSH04-09]
Catalog# HA722090
FBXO3 Recombinant Rabbit Monoclonal Antibody [PSH04-09]
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WB
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Human
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Mouse
-
unconjugated
Overview
Product Name
FBXO3 Recombinant Rabbit Monoclonal Antibody [PSH04-09]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human FBXO3 aa 1-450 / 471.
Species Reactivity
Human, Mouse
Validated Applications
WB
Target Molecular Weight
Predicted band size: 55 kDa
Positive Control
MDA-MB-231 cell lysate, HeLa cell lysate, K-562 cell lysate, MCF7 cell lysate, SK-Br-3 cell lysate, JAR cell lysate, HepG2 cell lysate, NIH/3T3 cell lysate.
Conjugation
unconjugated
Clone Number
PSH04-09
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
This gene encodes a member of the F-box protein family which is characterized by an approximately 40 amino acid motif, the F-box. The F-box proteins constitute one of the four subunits of the ubiquitin protein ligase complex called SCFs (SKP1-cullin-F-box), which function in phosphorylation-dependent ubiquitination. The F-box proteins are divided into 3 classes: Fbws containing WD-40 domains, Fbls containing leucine-rich repeats, and Fbxs containing either different protein-protein interaction modules or no recognizable motifs. The protein encoded by this gene belongs to the Fbxs class. Alternative splicing of this gene generates 2 transcript variants diverging at the 3' end.
Background References
1. Xu J et al. FBXO3 stabilizes USP4 and Twist1 to promote PI3K-mediated breast cancer metastasis. PLoS Biol. 2023 Dec
2. Gao Y et al. E3 Ubiquitin Ligase FBXO3 Drives Neuroinflammation to Aggravate Cerebral Ischemia/Reperfusion Injury. Int J Mol Sci. 2022 Nov
Subcellular Location
Nucleus.
Synonyms
F Box G - Domain 3 antibody
F box only protein 3 antibody
F box protein 3 antibody
F box protein FBX3 antibody
F-box only protein 3 antibody
FBA antibody
FBX3 antibody
FBX3_HUMAN antibody
FBXO3 antibody
Images
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Western blot analysis of FBXO3 on different lysates with Rabbit anti-FBXO3 antibody (HA722090) at 1/2,000 dilution.
Lane 1: MDA-MB-231 cell lysate
Lane 2: HeLa cell lysate
Lane 3: K-562 cell lysate
Lane 4: MCF7 cell lysate
Lane 5: SK-Br-3 cell lysate
Lane 6: JAR cell lysate
Lane 7: HepG2 cell lysate
Lane 8: NIH/3T3 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 55 kDa
Observed band size: 55/48 kDa
Exposure time: 1 minute 18 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722090) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"