SNX4 Recombinant Rabbit Monoclonal Antibody [JE65-39]
Catalog# HA722072
SNX4 Recombinant Rabbit Monoclonal Antibody [JE65-39]
-
WB
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
SNX4 Recombinant Rabbit Monoclonal Antibody [JE65-39]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within human SNX4 aa 351-400 / 450.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 52 kDa
Positive Control
HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, A431 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate.
Conjugation
unconjugated
Clone Number
JE65-39
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
Sorting nexin-4 is a protein that in humans is encoded by the SNX4 gene. This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein associated with the long isoform of the leptin receptor and with receptor tyrosine kinases for platelet-derived growth factor, insulin, and epidermal growth factor in cell cultures, but its function is unknown. This protein may form oligomeric complexes with family members. SNX4 has been shown to interact with BIN1.
Background References
1. Hanley SE et al. Snx4-assisted vacuolar targeting of transcription factors defines a new autophagy pathway for controlling ATG expression. Autophagy. 2021 Nov
2. Antón Z et al. A heterodimeric SNX4--SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly. J Cell Sci. 2020 Jul
Subcellular Location
Early endosome membrane.
Synonyms
ATG24B antibody
SNX 4 antibody
snx4 antibody
SNX4_HUMAN antibody
Sorting nexin 4 antibody
Sorting nexin-4 antibody
Images
-
Western blot analysis of SNX4 on different lysates with Rabbit anti-SNX4 antibody (HA722072) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate
Lane 2: HeLa cell lysate
Lane 3: Jurkat cell lysate
Lane 4: A431 cell lysate
Lane 5: RAW264.7 cell lysate
Lane 6: PC-12 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 52/36 kDa
Exposure time: 1 minute;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Rabbit IgG for IP Nano-secondary antibody (NBI01H) at 1/5,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"