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SNX4 Recombinant Rabbit Monoclonal Antibody [JE65-39]

Usd: 385

Specification

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Catalog# HA722072

SNX4 Recombinant Rabbit Monoclonal Antibody [JE65-39]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

SNX4 Recombinant Rabbit Monoclonal Antibody [JE65-39]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within human SNX4 aa 351-400 / 450.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Target Molecular Weight

Predicted band size: 52 kDa

Positive Control

HEK-293 cell lysate, HeLa cell lysate, Jurkat cell lysate, A431 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate.

Conjugation

unconjugated

Clone Number

JE65-39

RRID

AB_3096815

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Sorting nexin-4 is a protein that in humans is encoded by the SNX4 gene. This gene encodes a member of the sorting nexin family. Members of this family contain a phox (PX) domain, which is a phosphoinositide binding domain, and are involved in intracellular trafficking. This protein associated with the long isoform of the leptin receptor and with receptor tyrosine kinases for platelet-derived growth factor, insulin, and epidermal growth factor in cell cultures, but its function is unknown. This protein may form oligomeric complexes with family members. SNX4 has been shown to interact with BIN1.

Background References

1. Hanley SE et al. Snx4-assisted vacuolar targeting of transcription factors defines a new autophagy pathway for controlling ATG expression. Autophagy. 2021 Nov

2. Antón Z et al. A heterodimeric SNX4--SNX7 SNX-BAR autophagy complex coordinates ATG9A trafficking for efficient autophagosome assembly. J Cell Sci. 2020 Jul

Subcellular Location

Early endosome membrane.

UNIPROT

SwissProt: O95219 Human

SwissProt: Q91YJ2 Mouse

Entrez Gene: 360725 Rat

Synonyms

ATG24B antibody

SNX 4 antibody

snx4 antibody

SNX4_HUMAN antibody

Sorting nexin 4 antibody

Sorting nexin-4 antibody

Images

  • Western blot analysis of SNX4 on different lysates with Rabbit anti-SNX4 antibody (<a href="/products/HA722072" style="font-weight: bold;text-decoration: underline;">HA722072</a>) at 1/1,000 dilution.<br /><br />Lane 1: HEK-293 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: Jurkat cell lysate<br />Lane 4: A431 cell lysate<br />Lane 5: RAW264.7 cell lysate<br />Lane 6: PC-12 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 52 kDa<br />Observed band size: 52/36 kDa<br /><br />Exposure time: 1 minute;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722072" style="font-weight: bold;text-decoration: underline;">HA722072</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Rabbit IgG for IP, AlpSdAbs&reg; VHH(HRP) (<a href="/products/025-100-005" style="font-weight: bold;text-decoration: underline;">025-100-005</a>) at 1/5,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SNX4 on different lysates with Rabbit anti-SNX4 antibody (HA722072) at 1/1,000 dilution.

    Lane 1: HEK-293 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: Jurkat cell lysate
    Lane 4: A431 cell lysate
    Lane 5: RAW264.7 cell lysate
    Lane 6: PC-12 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 52 kDa
    Observed band size: 52/36 kDa

    Exposure time: 1 minute;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722072) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Anti-Rabbit IgG for IP, AlpSdAbs® VHH(HRP) (025-100-005) at 1/5,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"