Parathyroid Hormone Recombinant Rabbit Monoclonal Antibody [JE66-58]
Catalog# HA722059
Parathyroid Hormone Recombinant Rabbit Monoclonal Antibody [JE66-58]
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WB
-
IHC-P
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Human
-
Mouse
-
Rat
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unconjugated
Overview
Product Name
Parathyroid Hormone Recombinant Rabbit Monoclonal Antibody [JE66-58]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human Parathyroid Hormone aa 26-75 / 115.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P
Molecular Weight
Predicted band size: 13 kDa
Positive Control
HEK-293 cell lysate, CAL-62 cell lysate, A549 cell lysate, Jurkat cell lysate, mouse thymus tissue lysate, rat thymus tissue lysate, human parathyroid tissue.
Conjugation
unconjugated
Clone Number
JE66-58
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
-
IHC-P
-
1:100
Target
Function
Parathyroid hormone (PTH), also called parathormone or parathyrin, is a peptide hormone secreted by the parathyroid glands that regulates the serum calcium concentration through its effects on bone, kidney, and intestine. PTH influences bone remodeling, which is an ongoing process in which bone tissue is alternately resorbed and rebuilt over time. PTH is secreted in response to low blood serum calcium (Ca2+) levels. PTH indirectly stimulates osteoclast activity within the bone matrix (osteon), in an effort to release more ionic calcium (Ca2+) into the blood to elevate a low serum calcium level. The bones act as a (metaphorical) "bank of calcium" from which the body can make "withdrawals" as needed to keep the amount of calcium in the blood at appropriate levels despite the ever-present challenges of metabolism, stress, and nutritional variations. PTH is "a key that unlocks the bank vault" to remove the calcium. PTH is secreted primarily by the chief cells of the parathyroid glands. The gene for PTH is located on chromosome 11. It is a polypeptide containing 84 amino acids, which is a prohormone. It has a molecular mass around 9500 Da. Its action is opposed by the hormone calcitonin. Disorders that yield too little or too much PTH, such as hypoparathyroidism, hyperparathyroidism, and paraneoplastic syndromes can cause bone disease, hypocalcemia, and hypercalcemia.
Background References
1. Chen T et al. Parathyroid hormone and its related peptides in bone metabolism. Biochem Pharmacol. 2021 Oct
2. Kužma M et al. Parathyroid Hormone-Related Changes of Bone Structure. Physiol Res. 2021 Nov
Subcellular Location
Secreted.
Synonyms
hPTH antibody
Parathormone antibody
Parathyrin antibody
Parathyroid hormone 1 antibody
Parathyroid hormone antibody
Prepro PTH antibody
Preproparathyroid hormone antibody
PTH antibody
PTH1 antibody
PTH1 receptor antibody
ExpandImages
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Western blot analysis of Parathyroid Hormone on different lysates with Rabbit anti-Parathyroid Hormone antibody (HA722059) at 1/1,000 dilution.
Lane 1: HEK-293 cell lysate (20 µg/Lane)
Lane 2: CAL-62 cell lysate (20 µg/Lane)
Lane 3: A549 cell lysate (20 µg/Lane)
Lane 4: Jurkat cell lysate (20 µg/Lane)
Lane 5: Mouse thymus tissue lysate (40 µg/Lane)
Lane 6: Rat thymus tissue lysate (40 µg/Lane)
Predicted band size: 13 kDa
Observed band size: 16 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722059) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human parathyroid tissue with Rabbit anti-Parathyroid Hormone antibody (HA722059) at 1/100 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722059) at 1/100 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
Products with the same target and pathway
Parathyroid Hormone Recombinant Rabbit Monoclonal Antibody [PD00-92]
Application: IHC-P
Reactivity: Human
Conjugate: unconjugated
