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Calcipressin 1 Recombinant Rabbit Monoclonal Antibody [JE62-71]

Usd: 385

Specification

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Catalog# HA722057

Calcipressin 1 Recombinant Rabbit Monoclonal Antibody [JE62-71]

Application

  • WB

  • IF-Cell

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Calcipressin 1 Recombinant Rabbit Monoclonal Antibody [JE62-71]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Calcipressin 1 aa 50-150.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IF-Cell, FC

Molecular Weight

Predicted band size: 28 kDa

Positive Control

SW480 cell lysate, U-87 MG cell lysate, SH-SY5Y cell lysate, human brain tissue lysate, rat brain tissue lysate, mouse brain tissue lysate, mouse thymus tissue lysate, rat cerebellum tissue lysate, Jurkat.

Conjugation

unconjugated

Clone Number

JE62-71

RRID

AB_3096753

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

  • FC

  • 1:100

Target

Function

Down syndrome critical region gene 1, also known as DSCR1, is a protein that in humans is encoded by the RCAN1 gene. The protein encoded by this gene interacts with calcineurin A and inhibits calcineurin-dependent signaling pathways of genetic transcription, possibly affecting central nervous system development. Three transcript variants encoding three different isoforms have been found for this gene. In endothelial cells, VEGF stimulates RCAN1.4 expression which regulates gene expression, cell migration and tubular morphogenesis.

Background References

1. Lao M et al. RCAN1-mediated calcineurin inhibition as a target for cancer therapy. Mol Med. 2022 Jun

2. Wang S et al. RCAN1 in cardiovascular diseases: molecular mechanisms and a potential therapeutic target. Mol Med. 2020 Dec

Subcellular Location

Cytoplasm, nucleus.

UNIPROT

SwissProt: P53805 Human

SwissProt: Q9JHG6 Mouse

SwissProt: Q6IN33 Rat

Synonyms

Adapt 78 antibody

Adapt78 antibody

Calcipressin 1 antibody

Calcipressin-1 antibody

Calcium and oxidant inducible mRNA antibody

CSP 1 antibody

CSP1 antibody

Down syndrome candidate region 1 antibody

Down syndrome critical region gene 1 antibody

Down syndrome critical region protein 1 antibody

Expand

Images

  • Western blot analysis of Calcipressin 1 on different lysates with Rabbit anti-Calcipressin 1 antibody (<a href="/products/HA722057" style="font-weight: bold;text-decoration: underline;">HA722057</a>) at 1/1,000 dilution.<br /><br />Lane 1: SW480 cell lysate (20 µg/Lane)<br />Lane 2: U-87 MG cell lysate (20 µg/Lane)<br />Lane 3: SH-SY5Y cell lysate (20 µg/Lane)<br />Lane 4: Human brain tissue lysate (40 µg/Lane)<br />Lane 5: Rat brain tissue lysate (40 µg/Lane)<br />Lane 6: Mouse brain tissue lysate (40 µg/Lane)<br />Lane 7: Mouse thymus tissue lysate (40 µg/Lane)<br />Lane 8: Rat cerebellum tissue lysate (40 µg/Lane)<br /><br />Predicted band size: 28 kDa<br />Observed band size: 28 kDa<br /><br />Exposure time: 24 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722057" style="font-weight: bold;text-decoration: underline;">HA722057</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Calcipressin 1 on different lysates with Rabbit anti-Calcipressin 1 antibody (HA722057) at 1/1,000 dilution.

    Lane 1: SW480 cell lysate (20 µg/Lane)
    Lane 2: U-87 MG cell lysate (20 µg/Lane)
    Lane 3: SH-SY5Y cell lysate (20 µg/Lane)
    Lane 4: Human brain tissue lysate (40 µg/Lane)
    Lane 5: Rat brain tissue lysate (40 µg/Lane)
    Lane 6: Mouse brain tissue lysate (40 µg/Lane)
    Lane 7: Mouse thymus tissue lysate (40 µg/Lane)
    Lane 8: Rat cerebellum tissue lysate (40 µg/Lane)

    Predicted band size: 28 kDa
    Observed band size: 28 kDa

    Exposure time: 24 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722057) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Jurkat cells labeling Calcipressin 1 with Rabbit anti-Calcipressin 1 antibody (<a href="/products/HA722057" style="font-weight: bold;text-decoration: underline;">HA722057</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Calcipressin 1 antibody (<a href="/products/HA722057" style="font-weight: bold;text-decoration: underline;">HA722057</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of Jurkat cells labeling Calcipressin 1 with Rabbit anti-Calcipressin 1 antibody (HA722057) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Calcipressin 1 antibody (HA722057) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Flow cytometric analysis of Jurkat cells labeling Calcipressin 1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA722057" style="font-weight: bold;text-decoration: underline;">HA722057</a>, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of Jurkat cells labeling Calcipressin 1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA722057, 1/100) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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