Skip to content

Phospho-Chk1 (S317) Recombinant Rabbit Monoclonal Antibody [PSH03-84]

Usd: 385

Specification

Bulk Order Quote | Customization Request

Catalog# HA722050

Phospho-Chk1 (S317) Recombinant Rabbit Monoclonal Antibody [PSH03-84]

Application

  • WB

  • IHC-P

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

Phospho-Chk1 (S317) Recombinant Rabbit Monoclonal Antibody [PSH03-84]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phospho-peptide corresponding to residues surrounding Ser317 of Human Chk1.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P

Target Molecular Weight

Predicted band size: 54 kDa

Positive Control

HeLa cell lysate, HeLa treated with UV for 1 hour cell lysate, C6 cell lysate, C6 treated with 100nM Calyculin A for 30 minutes cell lysate, HeLa cells treated with or without UV for 40 minutes, NIH/3T3 cell lysate, NIH/3T3 treated with UV for 40 minutes add 1mM Sodium orthovanadate and recovery for 30 minutes cell lysate, L-929 treated with UV for 3 hours cell lysate.

Conjugation

unconjugated

Clone Number

PSH03-84

RRID

AB_3096843

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

Target

Function

Checkpoint kinases (Chks) are protein kinases that are involved in cell cycle control. Two checkpoint kinase subtypes have been identified, Chk1 and Chk2. Chk1 is a central component of genome surveillance pathways and is a key regulator of the cell cycle and cell survival. Chk1 is required for the initiation of DNA damage checkpoints and has recently been shown to play a role in the normal (unperturbed) cell cycle. Chk1 impacts various stages of the cell cycle including the S phase, G2/M transition and M phaase. In addition to mediating cell cycle checkpoints, Chk1 also contributes to DNA repair processes, gene transcription, egg production, embryo development, cellular responses to HIV infection and somatic cell viability.

Background References

1. Klomp JE et al. CHK1 protects oncogenic KRAS-expressing cells from DNA damage and is a target for pancreatic cancer treatment. Cell Rep. 2021 Nov

2. Zhu X et al. TRIM21 suppresses CHK1 activation by preferentially targeting CLASPIN for K63-linked ubiquitination. Nucleic Acids Res. 2022 Feb

Subcellular Location

Nucleus, Chromosome, Cytoplasm, cytoskeleton, microtubule organizing center, centrosome.

UNIPROT

SwissProt: O14757 Human

SwissProt: O35280 Mouse

SwissProt: Q91ZN7 Rat

Synonyms

C85740 antibody

Cell cycle checkpoint kinase antibody

Checkpoint , S. pombe, homolog of, 1 antibody

Checkpoint kinase 1 antibody

Checkpoint kinase 1 homolog (S. pombe) antibody

CHEK 1 antibody

Chek1 antibody

Chk 1 antibody

Chk1 antibody

CHK1 checkpoint homolog (S. pombe) antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-Chk1 (S317) on different lysates with Rabbit anti-Phospho-Chk1 (S317) antibody (<a href="/products/HA722050" style="font-weight: bold;text-decoration: underline;">HA722050</a>) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.<br /><br />Lane 1: HeLa cell lysate<br />Lane 2: HeLa treated with UV for 1 hour cell lysate<br />Lane 3: C6 cell lysate<br />Lane 4: C6 treated with 100nM Calyculin A for 30 minutes cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 43 seconds; ECL: K1802;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722050" style="font-weight: bold;text-decoration: underline;">HA722050</a>) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-Chk1 (S317) on different lysates with Rabbit anti-Phospho-Chk1 (S317) antibody (HA722050) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution.

    Lane 1: HeLa cell lysate
    Lane 2: HeLa treated with UV for 1 hour cell lysate
    Lane 3: C6 cell lysate
    Lane 4: C6 treated with 100nM Calyculin A for 30 minutes cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

    Exposure time: 43 seconds; ECL: K1802;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722050) at 1/1,000 dilution and competitor's antibody at 1/1,000 dilution were used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Immunohistochemical analysis of paraffin-embedded HeLa cells treated with or without UV for 40 minutes with Rabbit anti-Phospho-Chk1 (S317) antibody (<a href="/products/HA722050" style="font-weight: bold;text-decoration: underline;">HA722050</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722050" style="font-weight: bold;text-decoration: underline;">HA722050</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    ☑ Cell treatment (CT)

    Immunohistochemical analysis of paraffin-embedded HeLa cells treated with or without UV for 40 minutes with Rabbit anti-Phospho-Chk1 (S317) antibody (HA722050) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722050) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-Chk1 (S317) on different lysates with Rabbit anti-Phospho-Chk1 (S317) antibody (<a href="/products/HA722050" style="font-weight: bold;text-decoration: underline;">HA722050</a>) at 1/1,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate<br />Lane 2: NIH/3T3 treated with UV for 40 minutes add 1mM Sodium orthovanadate and recovery for 30 minutes cell lysate<br />Lane 3: L-929 cell lysate<br />Lane 4: L-929 treated with UV for 3 hours cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 54 kDa<br />Observed band size: 54 kDa<br /><br />Exposure time: 1 minute; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722050" style="font-weight: bold;text-decoration: underline;">HA722050</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-Chk1 (S317) on different lysates with Rabbit anti-Phospho-Chk1 (S317) antibody (HA722050) at 1/1,000 dilution.

    Lane 1: NIH/3T3 cell lysate
    Lane 2: NIH/3T3 treated with UV for 40 minutes add 1mM Sodium orthovanadate and recovery for 30 minutes cell lysate
    Lane 3: L-929 cell lysate
    Lane 4: L-929 treated with UV for 3 hours cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 54 kDa
    Observed band size: 54 kDa

    Exposure time: 1 minute; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722050) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Products with the same target and pathway

metafield image

Chk1 Recombinant Rabbit Monoclonal Antibody [JE00-39]

Application: WB,IF-Cell,IP

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Phospho-Chk1 (S345) Recombinant Rabbit Monoclonal Antibody [PS01-17] - BSA and Azide free

Application: WB,IF-Cell

Reactivity: Human,Mouse

Conjugate: unconjugated

metafield image

Phospho-Chk1 (S280) Recombinant Rabbit Monoclonal Antibody [JE47-66]

Application: WB

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Phospho-Chk1 (S296) Recombinant Rabbit Monoclonal Antibody [SN06-50]

Application: WB,IF-Cell

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated

metafield image

Chk1 Recombinant Rabbit Monoclonal Antibody [ST57-09] - BSA and Azide free

Application: WB,IF-Cell

Reactivity: Human

Conjugate: unconjugated

metafield image

Phospho-Chk1 (S345) Recombinant Rabbit Monoclonal Antibody [PS01-17]

Application: WB,IF-Cell

Reactivity: Human,Mouse

Conjugate: unconjugated

metafield image

Chk1 Recombinant Rabbit Monoclonal Antibody [ST57-09]

Application: WB,IF-Cell

Reactivity: Human

Conjugate: unconjugated

metafield image

Phospho-Chk1 (S317) Recombinant Rabbit Monoclonal Antibody [PSH03-84] - BSA and Azide free

Application: WB,IHC-P

Reactivity: Human,Mouse,Rat

Conjugate: unconjugated