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p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72]

Usd: 385

Specification

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Catalog# HA722036

p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

  • Green monkey

BSA and Azide free
Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72]

Antibody Type

Recombinant Rabbit multiclonal

Immunogen

Recombinant protein within human MAPK12 aa 1-367 / 367.

Product Specificity

Anti-p38 MAPK Antibody [PSH03-72] detects endogenous levels of total p38α, p38β, p38γ and p38δ MAPK protein.

Species Reactivity

Human, Mouse, Rat, Green monkey

Validated Applications

WB

Molecular Weight

Predicted band size: 42 kDa

Positive Control

293T cell lysate, HeLa cell lysate, K-562 cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, PC-12 cell lysate.

Conjugation

unconjugated

Clone Number

PSH03-72

RRID

AB_3096629

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

A mitogen-activated protein kinase (MAPK or MAP kinase) is a type of protein kinase that is specific to the amino acids serine and threonine (i.e., a serine/threonine-specific protein kinase). MAPKs are involved in directing cellular responses to a diverse array of stimuli, such as mitogens, osmotic stress, heat shock and proinflammatory cytokines. They regulate cell functions including proliferation, gene expression, differentiation, mitosis, cell survival, and apoptosis. MAP kinases are found in eukaryotes only, but they are fairly diverse and encountered in all animals, fungi and plants, and even in an array of unicellular eukaryotes. MAPKs belong to the CMGC (CDK/MAPK/GSK3/CLK) kinase group. The closest relatives of MAPKs are the cyclin-dependent kinases (CDKs). p38 MAP Kinase Antibody detects endogenous levels of total p38 alpha, p38 beta, p38 gamma, p38 delta protein.

Background References

1. Wang J et al. Pan-cancer analysis supports MAPK12 as a potential prognostic and immunotherapeutic target in multiple tumor types, including in THCA. Oncol Lett. 2022 Oct

2. Yang R et al. Prolactin Regulates Ovine Ovarian Granulosa Cell Apoptosis by Affecting the Expression of MAPK12 Gene. Int J Mol Sci. 2023 Jun

Subcellular Location

Cytoplasm, Nucleus, Mitochondrion.

UNIPROT

SwissProt: Q16539 Human

SwissProt: Q15759 Human

SwissProt: P53778 Human

SwissProt: O15264 Human

SwissProt: P47811 Mouse

SwissProt: Q9WUI1 Mouse

SwissProt: O08911 Mouse

SwissProt: Q9Z1B7 Mouse

SwissProt: P70618 Rat

SwissProt: D4A3U7 Rat

SwissProt: Q63538 Rat

SwissProt: Q9WTY9 Rat

Synonyms

ERK 6 antibody

ERK-6 antibody

ERK6 antibody

Extracellular signal-regulated kinase 6 antibody

MAP kinase 12 antibody

MAP kinase p38 gamma antibody

MAPK 12 antibody

Mapk12 antibody

Mitogen Activated Protein Kinase 12 antibody

Mitogen activated protein kinase p38 gamma antibody

Expand

Images

  • Western blot analysis of p38 MAPK on different lysates with Rabbit anti-p38 MAPK antibody (<a href="/products/HA722036" style="font-weight: bold;text-decoration: underline;">HA722036</a>) at 1/1,000 dilution.<br /><br />Lane 1: 293T cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: K-562 cell lysate<br />Lane 4: COS-1 cell lysate<br />Lane 5: Neuro-2a cell lysate<br />Lane 6: PC-12 cell lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 42 kDa<br />Observed band size: 42 kDa<br /><br />Exposure time: 4 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722036" style="font-weight: bold;text-decoration: underline;">HA722036</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of p38 MAPK on different lysates with Rabbit anti-p38 MAPK antibody (HA722036) at 1/1,000 dilution.

    Lane 1: 293T cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: K-562 cell lysate
    Lane 4: COS-1 cell lysate
    Lane 5: Neuro-2a cell lysate
    Lane 6: PC-12 cell lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 42 kDa
    Observed band size: 42 kDa

    Exposure time: 4 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722036) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation

Products with the same target and pathway

metafield image

p38 MAPK Recombinant Rabbit Multiclonal Antibody [PSH03-72] - BSA and Azide free

Application: WB

Reactivity: Human,Mouse,Rat,Green monkey

Conjugate: unconjugated