SP110 Recombinant Rabbit Monoclonal Antibody [PSH03-66]
Catalog# HA722025
SP110 Recombinant Rabbit Monoclonal Antibody [PSH03-66]
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WB
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IHC-P
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IF-Tissue
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Human
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Mouse
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Rat
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HA750900
不含抗保成分
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unconjugated
Overview
Product Name
SP110 Recombinant Rabbit Monoclonal Antibody [PSH03-66]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human SP110 aa 301-550 / 689.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB, IHC-P, IF-Tissue
Molecular Weight
Predicted band size: 78 kDa
Positive Control
Jurkat cell lysate, HeLa cell lysate, HL-60 cell lysate, SW480 cell lysate, Daudi cell lysate, K-562 cell lysate, PC-3M cell lysate, human lymph node tissue.
Conjugation
unconjugated
Clone Number
PSH03-66
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG1
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:2,000
Target
Function
SP110 nuclear body protein is a protein that in humans is encoded by the SP110 gene. The nuclear body is a multiprotein complex that may have a role in the regulation of gene transcription. This gene is a member of the SP100/SP140 family of nuclear body proteins and encodes a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator. In addition, it has been suggested that the protein may play a role in ribosome biogenesis and in the induction of myeloid cell differentiation. Alternative splicing has been observed for this gene and three transcript variants, encoding distinct isoforms, have been identified.
Background References
1. Cui X et al. Polymorphisms in the ASAP1 and SP110 Genes and Its Association with the Susceptibility to Pulmonary Tuberculosis in a Mongolian Population. J Immunol Res. 2022 Sep
2. Hamdoun O et al. Unique Mutation in SP110 Resulting in Hepatic Veno-Occlusive Disease with Immunodeficiency. Case Rep Pediatr. 2020 Jan
Subcellular Location
Nucleus.
UNIPROT
Synonyms
FLJ22835 antibody
IFI 41 antibody
IFI 75 antibody
IFI41 antibody
IFI75 antibody
Interferon induced protein 41 30kD antibody
Interferon induced protein 41 antibody
Interferon induced protein 41/75 antibody
Interferon induced protein 75 52kD antibody
Interferon induced protein 75 antibody
ExpandImages
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Western blot analysis of SP110 on different lysates with Rabbit anti-SP110 antibody (HA722025) at 1/1,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: HeLa cell lysate
Lane 3: HL-60 cell lysate
Lane 4: SW480 cell lysate
Lane 5: Daudi cell lysate
Lane 6: K-562 cell lysate
Lane 7: PC-3M cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 78 kDa
Observed band size: 29-78 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722025) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SP110 antibody (HA722025) at 1/2,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722025) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"