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SP110 Recombinant Rabbit Monoclonal Antibody [PSH03-66]

Usd: 385

Specification

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Catalog# HA722025

SP110 Recombinant Rabbit Monoclonal Antibody [PSH03-66]

Application

  • WB

  • IHC-P

  • IF-Tissue

Reactivity

  • Human

  • Mouse

  • Rat

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

SP110 Recombinant Rabbit Monoclonal Antibody [PSH03-66]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human SP110 aa 301-550 / 689.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, IF-Tissue

Target Molecular Weight

Predicted band size: 78 kDa

Positive Control

Jurkat cell lysate, HeLa cell lysate, HL-60 cell lysate, SW480 cell lysate, Daudi cell lysate, K-562 cell lysate, PC-3M cell lysate, human lymph node tissue.

Conjugation

unconjugated

Clone Number

PSH03-66

RRID

AB_3096688

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG1

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:2,000

Target

Function

SP110 nuclear body protein is a protein that in humans is encoded by the SP110 gene. The nuclear body is a multiprotein complex that may have a role in the regulation of gene transcription. This gene is a member of the SP100/SP140 family of nuclear body proteins and encodes a leukocyte-specific nuclear body component. The protein can function as an activator of gene transcription and may serve as a nuclear hormone receptor coactivator. In addition, it has been suggested that the protein may play a role in ribosome biogenesis and in the induction of myeloid cell differentiation. Alternative splicing has been observed for this gene and three transcript variants, encoding distinct isoforms, have been identified.

Background References

1. Cui X et al. Polymorphisms in the ASAP1 and SP110 Genes and Its Association with the Susceptibility to Pulmonary Tuberculosis in a Mongolian Population. J Immunol Res. 2022 Sep

2. Hamdoun O et al. Unique Mutation in SP110 Resulting in Hepatic Veno-Occlusive Disease with Immunodeficiency. Case Rep Pediatr. 2020 Jan

Subcellular Location

Nucleus.

UNIPROT

SwissProt: Q9HB58 Human

Synonyms

FLJ22835 antibody

IFI 41 antibody

IFI 75 antibody

IFI41 antibody

IFI75 antibody

Interferon induced protein 41 30kD antibody

Interferon induced protein 41 antibody

Interferon induced protein 41/75 antibody

Interferon induced protein 75 52kD antibody

Interferon induced protein 75 antibody

Expand

Images

  • Western blot analysis of SP110 on different lysates with Rabbit anti-SP110 antibody (<a href="/products/HA722025" style="font-weight: bold;text-decoration: underline;">HA722025</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: HL-60 cell lysate<br />Lane 4: SW480 cell lysate<br />Lane 5: Daudi cell lysate<br />Lane 6: K-562 cell lysate<br />Lane 7: PC-3M cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 78 kDa<br />Observed band size: 29-78 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA722025" style="font-weight: bold;text-decoration: underline;">HA722025</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of SP110 on different lysates with Rabbit anti-SP110 antibody (HA722025) at 1/1,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: HL-60 cell lysate
    Lane 4: SW480 cell lysate
    Lane 5: Daudi cell lysate
    Lane 6: K-562 cell lysate
    Lane 7: PC-3M cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 78 kDa
    Observed band size: 29-78 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722025) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SP110 antibody (<a href="/products/HA722025" style="font-weight: bold;text-decoration: underline;">HA722025</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA722025" style="font-weight: bold;text-decoration: underline;">HA722025</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human lymph node tissue with Rabbit anti-SP110 antibody (HA722025) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA722025) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"