EPCR Recombinant Rabbit Monoclonal Antibody [PSH03-50]
Catalog# HA722009
EPCR Recombinant Rabbit Monoclonal Antibody [PSH03-50]
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WB
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IF-Cell
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FC
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Human
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unconjugated
Overview
Product Name
EPCR Recombinant Rabbit Monoclonal Antibody [PSH03-50]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human EPCR aa 1-238 / 238.
Species Reactivity
Human
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 27 kDa
Positive Control
HUVEC cell lysate, EA.hy926 cell lysate, EA.hy926.
Conjugation
unconjugated
Clone Number
PSH03-50
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IF-Cell
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1:100
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FC
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1:1,000
Target
Function
The protein encoded by this gene is a receptor for activated protein C, a serine protease activated by and involved in the blood coagulation pathway. The encoded protein is an N-glycosylated type I membrane protein that enhances the activation of protein C. Mutations in this gene have been associated with venous thromboembolism and myocardial infarction, as well as with late fetal loss during pregnancy. The encoded protein may also play a role in malarial infection and has been associated with cancer. Binds activated protein C. Enhances protein C activation by the thrombin-thrombomodulin complex; plays a role in the protein C pathway controlling blood coagulation.
Background References
1. Ramadas N et al. The APC-EPCR-PAR1 axis in sickle cell disease. Front Med (Lausanne). 2023 Jul
2. Monroe D et al. EPCR knockout: inflaming the discussion. Blood. 2020 Jun 18
Subcellular Location
Membrane.
UNIPROT
Synonyms
Activated protein C receptor antibody
APC receptor antibody
APCR antibody
bA42O4.2 antibody
CCCA antibody
CCD41 antibody
CD201 antibody
CD201 antigen antibody
cell cycle, centrosome-associated protein antibody
Cell cycle, centrosome-associated protein antibody
ExpandImages
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☑ Relative expression (RE)
Western blot analysis of EPCR on different lysates with Rabbit anti-EPCR antibody (HA722009) at 1/1,000 dilution.
Lane 1: HUVEC cell lysate
Lane 2: EA.hy926 cell lysate
Lane 3: Jurkat cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 27 kDa
Observed band size: 40 kDa
Exposure time: 1 minute 2 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA722009) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Relative expression (RE)
Immunocytochemistry analysis of EA.hy926 (positive) and Jurkat (negative) labeling EPCR with Rabbit anti-EPCR antibody (HA722009) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-EPCR antibody (HA722009) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of EA.hy926 cells labeling EPCR.
Cells were washed twice with cold PBS and resuspend. Then stained with the primary antibody (HA722009, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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