DOCK2 Recombinant Rabbit Monoclonal Antibody [JE53-62]
Catalog# HA721993
DOCK2 Recombinant Rabbit Monoclonal Antibody [JE53-62]
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WB
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Human
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unconjugated
Overview
Product Name
DOCK2 Recombinant Rabbit Monoclonal Antibody [JE53-62]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human DOCK2 aa 1,781-1,830 / 1,830.
Species Reactivity
Human
Validated Applications
WB
Target Molecular Weight
Predicted band size: 212 kDa
Positive Control
Jurkat cell lysate, THP-1 cell lysate, Raji cell lysate.
Conjugation
unconjugated
Clone Number
JE53-62
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:2,000
Target
Function
Dedicator of cytokinesis protein 2 (Dock2) is a protein encoded in the human by the DOCK2 gene. Dock2 is a large (~180 kDa) protein involved in intracellular signalling networks. It is a member of the DOCK-A subfamily of the DOCK family of guanine nucleotide exchange factors (GEFs) which function as activators of small G-proteins. Dock2 specifically activates isoforms of the small G protein Rac.
Background References
1. Namkoong H et al. DOCK2 is involved in the host genetics and biology of severe COVID-19. Nature. 2022 Sep
2. Wang S et al. SULT2B1-CS-DOCK2 axis regulates effector T-cell exhaustion in HCC microenvironment. Hepatology. 2023 Oct
Subcellular Location
Endomembrane system, Cytoplasm, cytoskeleton.
UNIPROT
Synonyms
Dedicator of cyto kinesis 2 antibody
Dedicator of cytokinesis 2 antibody
Dedicator of cytokinesis protein 2 antibody
DOCK 2 antibody
FLJ46592 antibody
IMD40 antibody
KIAA0209 antibody
Images
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☑ Relative expression (RE)
Western blot analysis of DOCK2 on different lysates with Rabbit anti-DOCK2 antibody (HA721993) at 1/2,000 dilution.
Lane 1: Jurkat cell lysate
Lane 2: THP-1 cell lysate
Lane 3: Raji cell lysate
Lane 4: 293T cell lysate (negative)
Lysates/proteins at 20 µg/Lane.
Predicted band size: 212 kDa
Observed band size: 180 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721993) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"