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Serine racemase Recombinant Rabbit Monoclonal Antibody [JE65-58]

Usd: 385

Specification

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Catalog# HA721888

Serine racemase Recombinant Rabbit Monoclonal Antibody [JE65-58]

Application

  • WB

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

Serine racemase Recombinant Rabbit Monoclonal Antibody [JE65-58]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within Human Serine racemase aa 1-100 / 340.

Species Reactivity

Human

Validated Applications

WB

Target Molecular Weight

Predicted band size: 37 kDa

Positive Control

LNCaP cell lysate, U-87 MG cell lysate, HEK-293 cell lysate, MCF7 cell lysate, HeLa cell lysate, HepG2 cell lysate.

Conjugation

unconjugated

Clone Number

JE65-58

RRID

AB_3096683

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

Enables several functions, including L-serine ammonia-lyase activity; PDZ domain binding activity; and anion binding activity. Involved in pyruvate biosynthetic process; response to lipopolysaccharide; and serine family amino acid metabolic process. Located in cytoplasm and neuronal cell body. Catalyzes the synthesis of D-serine from L-serine. D-serine is a key coagonist with glutamate at NMDA receptors. Has dehydratase activity towards both L-serine and D-serine.

Background References

1. De Miranda J., Santoro A., Engelender S., Wolosker H. Human serine racemase: molecular cloning, genomic organization and functional expression. Gene 256:183-188 (2000)

2. Smith M.A., Mack V., Ebneth A., Moraes I., Felicetti B., Wood M., Schonfeld D., Mather O., Cesura A., Barker J. The structure of mammalian serine racemase: evidence for conformational changes upon inhibitor binding. J. Biol. Chem. 285:12873-12881 (2010)

Subcellular Location

Cytoplasm

UNIPROT

SwissProt: Q9GZT4 Human

Synonyms

D serine ammonia lyase antibody

D serine dehydratase antibody

D-serine ammonia-lyase antibody

D-serine dehydratase antibody

ILV1 antibody

ISO1 antibody

L serine ammonia lyase antibody

L serine dehydratase antibody

L-serine ammonia-lyase antibody

L-serine dehydratase antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of Serine racemase on different lysates with Rabbit anti-Serine racemase antibody (<a href="/products/HA721888" style="font-weight: bold;text-decoration: underline;">HA721888</a>) at 1/1,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-Serine racemase KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 37 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 120 seconds; ECL: K1801;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721888" style="font-weight: bold;text-decoration: underline;">HA721888</a>) at 1/1,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of Serine racemase on different lysates with Rabbit anti-Serine racemase antibody (HA721888) at 1/1,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-Serine racemase KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

    Exposure time: 120 seconds; ECL: K1801;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721888) at 1/1,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Western blot analysis of Serine racemase on different lysates with Rabbit anti-Serine racemase antibody (<a href="/products/HA721888" style="font-weight: bold;text-decoration: underline;">HA721888</a>) at 1/1,000 dilution.<br /><br />Lane 1: LNCaP cell lysate<br />Lane 2: U-87 MG cell lysate<br />Lane 3: HEK-293 cell lysate<br />Lane 4: MCF7 cell lysate<br />Lane 5: HeLa cell lysate<br />Lane 6: HepG2 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 37 kDa<br />Observed band size: 37 kDa<br /><br />Exposure time: 1 minute 59 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721888" style="font-weight: bold;text-decoration: underline;">HA721888</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of Serine racemase on different lysates with Rabbit anti-Serine racemase antibody (HA721888) at 1/1,000 dilution.

    Lane 1: LNCaP cell lysate
    Lane 2: U-87 MG cell lysate
    Lane 3: HEK-293 cell lysate
    Lane 4: MCF7 cell lysate
    Lane 5: HeLa cell lysate
    Lane 6: HepG2 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 37 kDa
    Observed band size: 37 kDa

    Exposure time: 1 minute 59 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721888) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"