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CD53 Recombinant Rabbit Monoclonal Antibody [JE60-38]

Usd: 385

Specification

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Catalog# HA721873

CD53 Recombinant Rabbit Monoclonal Antibody [JE60-38]

Application

  • WB

  • IF-Cell

  • IHC-P

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

CD53 Recombinant Rabbit Monoclonal Antibody [JE60-38]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human CD53 aa 170-219 / 219.

Species Reactivity

Human

Validated Applications

WB, IF-Cell, IHC-P

Target Molecular Weight

Predicted band size: 24 kDa

Positive Control

Jurkat cell lysate, Raji cell lysate, Ramos cell lysate, Jurkat, human tonsil tissue.

Conjugation

unconjugated

Clone Number

JE60-38

RRID

AB_3096759

Reactivity Data

Tested Tested Tested
Verified (internally validated)
Published Tested
Reported in literature (not internally validated)
Predicted Tested
Predicted reactive (based on sequence homology)
Not recommended Tested
Not recommended (failed internal validation)
WB IHC-P FC IF-Cell
human
Tested Tested
Tested Tested
Not Recommended
Tested Tested
mouse
Not Recommended
 
 
 
rat
Not Recommended
 
 
 

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IF-Cell

  • 1:100

  • IHC-P

  • 1:2,000

Target

Function

Leukocyte surface antigen CD53 is a protein that in humans is encoded by the CD53 gene. The protein encoded by this gene is a member of the transmembrane 4 superfamily, also known as the tetraspanin family. Most of these members are cell-surface proteins that are characterized by the presence of four hydrophobic domains. The proteins mediate signal transduction events that play a role in the regulation of cell development, activation, growth and motility. This encoded protein is a cell surface glycoprotein that is known to complex with integrins. It contributes to the transduction of CD2-generated signals in T cells and natural killer cells and has been suggested to play a role in growth regulation. Familial deficiency of this gene has been linked to an immunodeficiency associated with recurrent infectious diseases caused by bacteria, fungi and viruses. Alternative splicing results in multiple transcript variants encoding the same protein.

Background References

1. Karabacz N et al. CD53 sends HSCs to sweet DREAMs. Blood. 2023 Mar

2. Demaria MC et al. Tetraspanin CD53 Promotes Lymphocyte Recirculation by Stabilizing L-Selectin Surface Expression. iScience. 2020 May

Subcellular Location

Cell membrane, Cell junction, Membrane.

UNIPROT

SwissProt: P19397 Human

Synonyms

AI323659 antibody

Antigen MOX44 identified by monoclonal antibody MRC-OX44 antibody

CD 53 antibody

CD53 antibody

CD53 antigen antibody

CD53 antigen, isoform CRA_b antibody

CD53 glycoprotein antibody

Cd53 molecule antibody

CD53 tetraspan antigen antibody

CD53_HUMAN antibody

Expand

Images

  • Western blot analysis of CD53 on different lysates with Rabbit anti-CD53 antibody (<a href="/products/HA721873" style="font-weight: bold;text-decoration: underline;">HA721873</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: Raji cell lysate<br />Lane 3: Ramos cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 24 kDa<br />Observed band size: 35-42 kDa<br /><br />Exposure time: 1 minute 14 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721873" style="font-weight: bold;text-decoration: underline;">HA721873</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of CD53 on different lysates with Rabbit anti-CD53 antibody (HA721873) at 1/1,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: Raji cell lysate
    Lane 3: Ramos cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 24 kDa
    Observed band size: 35-42 kDa

    Exposure time: 1 minute 14 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721873) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of Jurkat cells labeling CD53 with Rabbit anti-CD53 antibody (<a href="/products/HA721873" style="font-weight: bold;text-decoration: underline;">HA721873</a>) at 1/100 dilution.<br /><br />Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD53 antibody (<a href="/products/HA721873" style="font-weight: bold;text-decoration: underline;">HA721873</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of Jurkat cells labeling CD53 with Rabbit anti-CD53 antibody (HA721873) at 1/100 dilution.

    Cells were fixed in 80% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-CD53 antibody (HA721873) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD53 antibody (<a href="/products/HA721873" style="font-weight: bold;text-decoration: underline;">HA721873</a>) at 1/2,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721873" style="font-weight: bold;text-decoration: underline;">HA721873</a>) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human tonsil tissue with Rabbit anti-CD53 antibody (HA721873) at 1/2,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721873) at 1/2,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"