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Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) Recombinant Rabbit Monoclonal Antibody [JE45-17]

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Specification

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Catalog# HA721864

Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) Recombinant Rabbit Monoclonal Antibody [JE45-17]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) Recombinant Rabbit Monoclonal Antibody [JE45-17]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Thr197 of PKA C-gamma.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 40 kDa

Positive Control

NIH/3T3 cell lysate, C6 cell lysate, HeLa cell lysate, HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate.

Conjugation

unconjugated

Clone Number

JE45-17

RRID

AB_3072974

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

The catalytic subunit α of protein kinase A is a key regulatory enzyme that in humans is encoded by the PRKACA gene. cAMP-dependent protein kinase catalytic subunit beta is an enzyme that in humans is encoded by the PRKACB gene. cAMP-dependent protein kinase catalytic subunit gamma is an enzyme that in humans is encoded by the PRKACG gene. cAMP is a signaling molecule important for a variety of cellular functions. cAMP exerts its effects by activating the protein kinase A (PKA), which transduces the signal through phosphorylation of different target proteins. The inactive holoenzyme of PKA is a tetramer composed of two regulatory and two catalytic subunits. cAMP causes the dissociation of the inactive holoenzyme into a dimer of regulatory subunits bound to four cAMP and two free monomeric catalytic subunits. Four different regulatory subunits and three catalytic subunits of PKA have been identified in humans. Defective regulation of PKA holoenzyme activity has been linked to the progression of cardiovascular disease, certain endocrine disorders and cancers.

Background References

1. Bauer J et al. The oncogenic fusion protein DNAJB1-PRKACA can be specifically targeted by peptide-based immunotherapy in fibrolamellar hepatocellular carcinoma. Nat Commun. 2022 Oct

2. Palencia-Campos A et al. Germline and Mosaic Variants in PRKACA and PRKACB Cause a Multiple Congenital Malformation Syndrome. Am J Hum Genet. 2020 Nov

Subcellular Location

Cytoplasm, Cell membrane, Nucleus.

UNIPROT

SwissProt: P17612 Human

SwissProt: P22694 Human

SwissProt: P22612 Human

SwissProt: P05132 Mouse

SwissProt: P27791 Rat

Synonyms

cAMP dependent protein kinase catalytic subunit alpha antibody

cAMP dependent protein kinase catalytic subunit beta antibody

cAMP dependent protein kinase catalytic subunit gamma antibody

PKA C alpha antibody

PKA C beta antibody

PKA C gamma antibody

PKACA antibody

PRKACA antibody

PRKACB antibody

PRKACG antibody

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) on different lysates with Rabbit anti-Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) antibody (<a href="/products/HA721864" style="font-weight: bold;text-decoration: underline;">HA721864</a>) at 1/1,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate<br />Lane 2: C6 cell lysate<br />Lane 3: HeLa cell lysate<br />Lane 4: HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 40 kDa<br />Observed band size: 40 kDa<br /><br />Exposure time: 46 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721864" style="font-weight: bold;text-decoration: underline;">HA721864</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) on different lysates with Rabbit anti-Phospho-PKA alpha/beta/gamma (catalytic subunit) (T197) antibody (HA721864) at 1/1,000 dilution.

    Lane 1: NIH/3T3 cell lysate
    Lane 2: C6 cell lysate
    Lane 3: HeLa cell lysate
    Lane 4: HeLa serum starved overnight then treated with 100nM Calyculin A for 1 hour cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 40 kDa
    Observed band size: 40 kDa

    Exposure time: 46 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721864) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation