NSUN6 Recombinant Rabbit Monoclonal Antibody [PSH02-80]
Catalog# HA721858
NSUN6 Recombinant Rabbit Monoclonal Antibody [PSH02-80]
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WB
-
Human
-
Mouse
-
Rat
-
unconjugated
Overview
Product Name
NSUN6 Recombinant Rabbit Monoclonal Antibody [PSH02-80]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human NSUN6 aa 1-469 / 469.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Target Molecular Weight
Predicted band size: 52 kDa
Positive Control
HeLa cell lysate, HEK-293 cell lysate, HepG2 cell lysate, K-562 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, rat pancreas tissue lysate.
Conjugation
unconjugated
Clone Number
PSH02-80
RRID
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
Target
Function
S-adenosyl-L-methionine-dependent methyltransferase that specifically methylates the C5 position of cytosine 72 in tRNA(Thr)(TGT) and tRNA(Cys)(GCA). In vitro also methylates tRNA(Thr)(AGT). Methylation requires, in the acceptor stem region, the presence of the 3'-CCA terminus, the target site C72, the discriminator base U73, and the second and third base pairs (2:71 and 3:70) in the tRNA substrates.
Background References
1. Yang R et al. The RNA methyltransferase NSUN6 suppresses pancreatic cancer development by regulating cell proliferation. EBioMedicine. 2021 Jan
2. Selmi T et al. Sequence- and structure-specific cytosine-5 mRNA methylation by NSUN6. Nucleic Acids Res. 2021 Jan
Subcellular Location
Cytoplasm.
Synonyms
4933414E04Rik antibody
FLJ23743 antibody
NOL1/NOP2/Sun and PUA domain-containing protein 1 antibody
NOL1/NOP2/Sun domain family 6 antibody
NOL1/NOP2/Sun domain family member 6 antibody
NOP2 antibody
NOPD1 antibody
NSUN6 antibody
NSUN6_HUMAN antibody
nucleolar protein (NOL1/NOP2/sun) and PUA domains 1 antibody
ExpandImages
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Western blot analysis of NSUN6 on different lysates with Rabbit anti-NSUN6 antibody (HA721858) at 1/2,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HEK-293 cell lysate
Lane 3: HepG2 cell lysate
Lane 4: K-562 cell lysate
Lane 5: Jurkat cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: Rat pancreas tissue lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 52 kDa
Observed band size: 52 kDa
Exposure time: 1 minute 58 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721858) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"