Human IL-12B p40 Recombinant Rabbit Monoclonal Antibody [PSH02-58] - BSA and Azide free (Detector)
Overview
Product Name
Human IL-12B p40 Recombinant Rabbit Monoclonal Antibody [PSH02-58] - BSA and Azide free (Detector)
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human IL12B/P40 aa 23-328 (P29460).
Species Reactivity
Human, Dog, Cynomolgus monkey
Validated Applications
ELISA(Det)
Positive Control
Recombinant Human IL-12B p40 protein.
Conjugation
unconjugated
Clone Number
PSH02-58
RRID
Product Features
Form
Liquid
Concentration
1 mg/ml(The concentration of this product may be batch-dependent)
Storage Instructions
Store at 2-8℃. Avoid freeze.
Storage Buffer
PBS (pH7.4).
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
ELISA(Det)
-
Use at an assay dependent concentration. Can be paired for Sandwich ELISA with Rabbit monoclonal [PSH02-57] to Human IL-12B p40 (Capture) (HA721834) and recombinant human 12B p40 protein (HA210655) as the standard. The reference range value is 30.9-2,500 pg/mL.
Target
Function
Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC. Associates with IL23A to form the IL-23 interleukin, a heterodimeric cytokine which functions in innate and adaptive immunity. IL-23 may constitute with IL-17 an acute response to infection in peripheral tissues. IL-23 binds to a heterodimeric receptor complex composed of IL12RB1 and IL23R, activates the Jak-Stat signaling cascade, stimulates memory rather than naive T-cells and promotes production of pro-inflammatory cytokines. IL-23 induces autoimmune inflammation and thus may be responsible for autoimmune inflammatory diseases and may be important for tumorigenesis.
Background References
1. Oppmann B., Lesley R., Blom B., Timans J.C., Xu Y., Hunte B., Vega F., Yu N., Wang J., Kastelein R.A. Novel p19 protein engages IL-12p40 to form a cytokine, IL-23, with biological activities similar as well as distinct from IL-12. Immunity 13:715-725 (2000)
Subcellular Location
Secreted
UNIPROT
Synonyms
CLMF antibody
CLMF p40 antibody
CLMF2 antibody
Cytotoxic lymphocyte maturation factor 40 kDa subunit antibody
IL 12 subunit p40 antibody
IL 12B antibody
IL-12 subunit p40 antibody
IL-12B antibody
IL12 p40 antibody
IL12 subunit p40 antibody
ExpandImages
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![Sandwich ELISA analysis of human IL-12B p40 matched pair antibodies.<br /><br />Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (<a href="/products/HA721834" style="font-weight: bold;text-decoration: underline;">HA721834</a>) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human IL-12B p40 protein (<a href="/products/HA210655" style="font-weight: bold;text-decoration: underline;">HA210655</a>) starting from 2500 pg/ml to 0 pg/ml and detect antibody [PSH02-58] Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.](https://storage.huabio.cn/huabio/productImg/HA721835_1.jpg?v=20260908003423)
Sandwich ELISA analysis of human IL-12B p40 matched pair antibodies.
Elisa assay was performed by coating wells of a 96-well plate with 100 µl per well of capture antibody (HA721834) diluted in carbonate/bicarbonate buffer, at a concentration of 2 µg/ml overnight at 4℃. Wells of the plate were washed, blocked with 150 µl 0.05% tween-20 1%BSA blocking buffer, and incubated with serial diluted human IL-12B p40 protein (HA210655) starting from 2500 pg/ml to 0 pg/ml and detect antibody [PSH02-58] Biotin for 1 hour at 30℃ with shaking. Then the plate was washed and incubated with 100 µl per well of SA-HRP for 0.5 hour at 30℃ with shaking. Detection was performed using an Ultra TMB Substrate for 10 minutes at room temperature in the dark. The reaction was stopped with sulfuric acid and absorbances were read on a spectrophotometer at 450 nm.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"
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Biotin Conjugated Human IL-12B p40 Recombinant Rabbit Monoclonal Antibody [PSH02-58] - Detector
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Reactivity: Human,Dog,Cynomolgus monkey
Conjugate: Biotin
Biotin Conjugated Human IL-12B p40 Recombinant Rabbit Monoclonal Antibody [PSH03-04]
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Human IL-12B p40 Recombinant Rabbit Monoclonal Antibody [PSH03-04] - BSA and Azide free (Detector)
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