Merlin Recombinant Rabbit Monoclonal Antibody [PSH02-52]
Catalog# HA721828
Merlin Recombinant Rabbit Monoclonal Antibody [PSH02-52]
-
WB
-
IF-Cell
-
FC
-
Human
-
Mouse
-
Rat
-
Green monkey
-
unconjugated
Overview
Product Name
Merlin Recombinant Rabbit Monoclonal Antibody [PSH02-52]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human Merlin aa 1-350 / 595 (P35240).
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IF-Cell, FC
Target Molecular Weight
Predicted band size: 70 kDa
Positive Control
PC-3M cell lysate, HeLa cell lysate, MDA-MB-468 cell lysate, SH-SY5Y cell lysate, HUVEC cell lysate, HEK-293 cell lysate, Jurkat cell lysate, COS-1 cell lysate, Neuro-2a cell lysate, NIH/3T3 cell lysate, C6 cell lysate, HUVEC, NIH/3T3.
Conjugation
unconjugated
Clone Number
PSH02-52
RRID
Reactivity Data
| WB | IF-Cell | FC | |
|---|---|---|---|
| Human |
|
|
|
| Mouse |
|
|
|
| Rat |
|
||
| Green Monkey |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:2,000
-
IF-Cell
-
1:100
-
FC
-
1:1,000
Target
Function
Merlin (also called Neurofibromin 2 or schwannomin) is a cytoskeletal protein. In humans, it is a tumor suppressor protein involved in neurofibromatosis type II. Sequence data reveal its similarity to the ERM protein family. The name "merlin" is an acronym for "Moesin-Ezrin-Radixin-Like Protein". Merlin is a membrane-cytoskeleton scaffolding protein, i.e. linking actin filaments to cell membrane or membrane glycoproteins. Human merlin is predominantly found in nervous tissue, but also in several other fetal tissues, and is mainly located in adherens junctions. Its tumor suppressor properties are probably associated with contact-mediated growth inhibition. Drosophila merlin is expressed in embryonic hindgut, salivary glands, and imaginal discs, and has apparently a slightly different role than in vertebrates. The phosphorylation of serine 518 is known to alter the functional state of merlin. The signaling pathway of merlin is proposed to include several salient cell growth controlling molecules, including eIF3c, CD44, protein kinase A, and p21 activated kinases. Mutations of the NF2 gene cause a human autosomal dominant disease called neurofibromatosis type 2. It is characterized by the development of tumors of the nervous system, most commonly of bilateral vestibular schwannomas (also called acoustic neuromas). NF2 belongs to the tumor suppressor group of genes.
Background References
1. Mota M et al. Merlin regulates signaling events at the nexus of development and cancer. Cell Commun Signal. 2020 Apr
2. Zhou Z et al. Merlin functions as a critical regulator in Staphylococcus aureus-induced osteomyelitis. J Cell Physiol. 2022 Jan
Subcellular Location
Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane, Nucleus.
Synonyms
ACN antibody
BANF antibody
Bilateral acoustic neuroma antibody
MERL_HUMAN antibody
Merlin antibody
Moesin ezrin radixin like protein antibody
Moesin ezrin radizin like antibody
Moesin-ezrin-radixin-like protein antibody
Neurofibromatosis 2 antibody
Neurofibromatosis type 2 antibody
ExpandImages
-
☑ Relative expression (RE)
Western blot analysis of Merlin on different lysates with Rabbit anti-Merlin antibody (HA721828) at 1/2,000 dilution.
Lane 1: PC-3M cell lysate (20 µg/Lane)
Lane 2: HeLa cell lysate (20 µg/Lane)
Lane 3: MDA-MB-468 cell lysate (20 µg/Lane)
Lane 4: SH-SY5Y cell lysate (20 µg/Lane)
Lane 5: HUVEC cell lysate (20 µg/Lane)
Lane 6: HEK-293 cell lysate (20 µg/Lane)
Lane 7: Jurkat cell lysate (20 µg/Lane)
Lane 8: MDA-MB-231 cell lysate (negative) (20 µg/Lane)
Lane 9: COS-1 cell lysate (20 µg/Lane)
Lane 10: Neuro-2a cell lysate (20 µg/Lane)
Lane 11: NIH/3T3 cell lysate (20 µg/Lane)
Lane 12: C6 cell lysate (20 µg/Lane)
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 1 minute 41 seconds; ECL: K1802;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721828) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
☑ Knockdown (KD)
Western blot analysis of Merlin on different lysates with Rabbit anti-Merlin antibody (HA721828) at 1/2,000 dilution.
Lane 1: HCT 116-si NT cell lysate
Lane 2: HCT 116-si Merlin cell lysate
Lysates/proteins at 10 µg/Lane.
Predicted band size: 70 kDa
Observed band size: 70 kDa
Exposure time: 30 seconds; ECL: K1801;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721828) at 1/2,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HUVEC cells labeling Merlin with Rabbit anti-Merlin antibody (HA721828) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Merlin antibody (HA721828) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunocytochemistry analysis of NIH/3T3 cells labeling Merlin with Rabbit anti-Merlin antibody (HA721828) at 1/100 dilution.
Cells were fixed in 100% precooled methanol for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Merlin antibody (HA721828) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Flow cytometric analysis of NIH/3T3 cells labeling Merlin.
Cells were fixed and permeabilized. Then stained with the primary antibody (HA721828, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"