Transcription factor AP-2-alpha Recombinant Rabbit Monoclonal Antibody [PSH02-06]
Catalog# HA721765
Transcription factor AP-2-alpha Recombinant Rabbit Monoclonal Antibody [PSH02-06]
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WB
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IHC-P
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IF-Cell
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Human
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unconjugated
Overview
Product Name
Transcription factor AP-2-alpha Recombinant Rabbit Monoclonal Antibody [PSH02-06]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within human TFAP2A aa 1-437 / 437.
Species Reactivity
Human
Validated Applications
WB, IHC-P, IF-Cell
Target Molecular Weight
Predicted band size: 48 kDa
Positive Control
HepG2 cell lysate, SiHa cell lysate, HepG2, human breast tissue.
Conjugation
unconjugated
Clone Number
PSH02-06
RRID
Reactivity Data
| WB | IHC-P | IF-Cell | |
|---|---|---|---|
| Human |
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| Mouse |
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| Rat |
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Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
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WB
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1:1,000
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IHC-P
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1:1,000
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IF-Cell
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1:100
Target
Function
Sequence-specific DNA-binding protein that interacts with inducible viral and cellular enhancer elements to regulate transcription of selected genes. AP-2 factors bind to the consensus sequence 5'-GCCNNNGGC-3' and activate genes involved in a large spectrum of important biological functions including proper eye, face, body wall, limb and neural tube development. They also suppress a number of genes including MCAM/MUC18, C/EBP alpha and MYC. AP-2-alpha is the only AP-2 protein required for early morphogenesis of the lens vesicle. Together with the CITED2 coactivator, stimulates the PITX2 P1 promoter transcription activation. Associates with chromatin to the PITX2 P1 promoter region.
Background References
1. Liao H et al. The comprehensive investigation of transcription factor AP-2 alpha in lung adenocarcinoma. Transl Cancer Res. 2020 Mar
2. Cui Y et al. TFAP2A-induced SLC2A1-AS1 promotes cancer cell proliferation. Biol Chem. 2021 Feb
Subcellular Location
Nucleus.
UNIPROT
Synonyms
Activating enhancer binding protein 2 alpha antibody
Activating enhancer-binding protein 2-alpha antibody
Activator protein 2 antibody
AP 2 transcription factor antibody
AP 2alpha antibody
AP-2 antibody
AP-2 transcription factor antibody
AP2 antibody
AP2 Transcription Factor antibody
AP2-alpha antibody
ExpandImages
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Western blot analysis of Transcription factor AP-2-alpha on different lysates with Rabbit anti-Transcription factor AP-2-alpha antibody (HA721765) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: SiHa cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 48 kDa
Observed band size: 48 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721765) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature. -
Immunocytochemistry analysis of HepG2 cells labeling Transcription factor AP-2-alpha with Rabbit anti-Transcription factor AP-2-alpha antibody (HA721765) at 1/100 dilution.
Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Transcription factor AP-2-alpha antibody (HA721765) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.
Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution. -
Immunohistochemical analysis of paraffin-embedded human breast tissue with Rabbit anti-Transcription factor AP-2-alpha antibody (HA721765) at 1/1,000 dilution.
The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721765) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"