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FLIP Recombinant Rabbit Monoclonal Antibody [PSH01-95]

Usd: 385

Specification

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Catalog# HA721753

FLIP Recombinant Rabbit Monoclonal Antibody [PSH01-95]

Application

  • WB

Reactivity

  • Human

BSA and Azide free
Conjugation

  • unconjugated

Overview

Product Name

FLIP Recombinant Rabbit Monoclonal Antibody [PSH01-95]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant protein within human FLIP aa 1-480 / 480.

Species Reactivity

Human

Validated Applications

WB

Target Molecular Weight

Predicted band size: 55 kDa

Positive Control

A549 cell lysate, HeLa cell lysate, 293T cell lysate, K-562 cell lysate, HCT 116 cell lysate, MDA-MB-468 cell lysate, MDA-MB-231 cell lysate, Jurkat cell lysate, SW480 cell lysate.

Conjugation

unconjugated

Clone Number

PSH01-95

RRID

AB_3072865

Reactivity Data

WB
Human
Tested Tested
Mouse
Not Recommended
Rat
Not Recommended

Product Features

Form

Liquid

Concentration

1 mg/mL.(The concentration of this product may be batch-dependent)

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

The protein encoded by this gene is a regulator of apoptosis and is structurally similar to caspase-8. However, the encoded protein lacks caspase activity and appears to be itself cleaved into two peptides by caspase-8. Several transcript variants encoding different isoforms have been found for this gene, and partial evidence for several more variants exists.

Background References

1. Smyth P et al. FLIP(L): the pseudo-caspase. FEBS J. 2020 Oct

2. Kitzmann WR et al. Spin-flip luminescence. Photochem Photobiol Sci. 2022 Jul

Subcellular Location

Cytoplasm, cytosol.

UNIPROT

SwissProt: O15519 Human

Synonyms

c FLIP antibody

c FLIPL antibody

c FLIPR antibody

c FLIPS antibody

c-FLIP antibody

c-FLIPL antibody

c-FLIPR antibody

c-FLIPS antibody

CASH antibody

CASP8 and FADD like apoptosis regulator antibody

Expand

Images

  • Western blot analysis of FLIP on different lysates with Rabbit anti-FLIP antibody (<a href="/products/HA721753" style="font-weight: bold;text-decoration: underline;">HA721753</a>) at 1/1,000 dilution.<br /><br />Lane 1: A549 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: 293T cell lysate<br />Lane 4: K-562 cell lysate<br />Lane 5: HCT 116 cell lysate<br />Lane 6: MDA-MB-468 cell lysate<br />Lane 7: MDA-MB-231 cell lysate<br />Lane 8: Jurkat cell lysate<br />Lane 9: SW480 cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 55 kDa<br />Observed band size: 55/25 kDa<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721753" style="font-weight: bold;text-decoration: underline;">HA721753</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of FLIP on different lysates with Rabbit anti-FLIP antibody (HA721753) at 1/1,000 dilution.

    Lane 1: A549 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: 293T cell lysate
    Lane 4: K-562 cell lysate
    Lane 5: HCT 116 cell lysate
    Lane 6: MDA-MB-468 cell lysate
    Lane 7: MDA-MB-231 cell lysate
    Lane 8: Jurkat cell lysate
    Lane 9: SW480 cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 55 kDa
    Observed band size: 55/25 kDa

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721753) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"