eIF4A2 Recombinant Rabbit Monoclonal Antibody [PSH01-89]
Catalog# HA721747
eIF4A2 Recombinant Rabbit Monoclonal Antibody [PSH01-89]
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WB
-
IP
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Human
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Mouse
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Rat
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Green monkey
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unconjugated
Overview
Product Name
eIF4A2 Recombinant Rabbit Monoclonal Antibody [PSH01-89]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human eIF4A2 aa 1-407 / 407.
Species Reactivity
Human, Mouse, Rat, Green monkey
Validated Applications
WB, IP
Target Molecular Weight
Predicted band size: 46 kDa
Positive Control
HepG2 cell lysate, HeLa cell lysate, PC-3M cell lysate, 293T cell lysate, MCF7 cell lysate, NIH/3T3 cell lysate, C2C12 cell lysate, PC-12 cell lysate, COS-1 cell lysate, human brain tissue lysate, mouse testis tissue lysate, rat testis tissue lysate.
Conjugation
unconjugated
Clone Number
PSH01-89
RRID
Reactivity Data
| WB | IP | |
|---|---|---|
| Human |
|
|
| Mouse |
|
|
| Rat |
|
|
| Green Monkey |
|
Product Features
Form
Liquid
Concentration
1 mg/mL.(The concentration of this product may be batch-dependent)
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
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IP
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1:1,000
Target
Function
ATP-dependent RNA helicase which is a subunit of the eIF4F complex involved in cap recognition and is required for mRNA binding to ribosome. In the current model of translation initiation, eIF4A unwinds RNA secondary structures in the 5'-UTR of mRNAs which is necessary to allow efficient binding of the small ribosomal subunit, and subsequent scanning for the initiator codon.
Background References
1. Liu L. et al. 2022.The splicing factor RBM17 drives leukemic stem cell maintenance by evading nonsense-mediated decay of pro-leukemic factors. Nat Commun. 13(1):3833.
2. Chen Z.H. et al. 2019. Eukaryotic initiation factor 4A2 promotes experimental metastasis and oxaliplatin resistance in colorectal cancer. J Exp Clin Cancer Res.38(1):196.
Subcellular Location
Cytosol.
Synonyms
ATP dependent RNA helicase eIF4A 2 antibody
ATP-dependent RNA helicase eIF4A-2 antibody
BM-010 antibody
DDX2B antibody
eIF 4A II antibody
eIF-4A-II antibody
EIF4A antibody
eIF4A II antibody
eIF4A-II antibody
EIF4A2 antibody
ExpandImages
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Western blot analysis of eIF4A2 on different lysates with Rabbit anti-eIF4A2 antibody (HA721747) at 1/1,000 dilution.
Lane 1: HepG2 cell lysate
Lane 2: HeLa cell lysate
Lane 3: PC-3M cell lysate
Lane 4: 293T cell lysate
Lane 5: MCF7 cell lysate
Lane 6: NIH/3T3 cell lysate
Lane 7: C2C12 cell lysate
Lane 8: PC-12 cell lysate
Lane 9: COS-1 cell lysate
Lane 10: Human brain tissue lysate
Lane 11: Mouse testis tissue lysate
Lane 12: Rat testis tissue lysate
Lysates/proteins at 30 µg/Lane.
Predicted band size: 46 kDa
Observed band size: 46 kDa
Exposure time: 24 seconds;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721747) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"