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TIMP1 Recombinant Rabbit Monoclonal Antibody [JE59-72]

Usd: 385

Specification

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Catalog# HA721741

TIMP1 Recombinant Rabbit Monoclonal Antibody [JE59-72]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

Conjugation

  • unconjugated

Overview

Product Name

TIMP1 Recombinant Rabbit Monoclonal Antibody [JE59-72]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Recombinant full length protein within human TIMP1.

Species Reactivity

Human

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 23 kDa

Positive Control

HT-29 cell lysate, PANC-1 cell lysate, SW1990 cell lysate, human pancreas tissue, HT-29.

Conjugation

unconjugated

Clone Number

JE59-72

RRID

AB_3072853

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • FC

  • 1:1,000

Target

Function

This gene belongs to the TIMP gene family. The proteins encoded by this gene family are natural inhibitors of the matrix metalloproteinases (MMPs), a group of peptidases involved in degradation of the extracellular matrix. In addition to its inhibitory role against most of the known MMPs, the encoded protein is able to promote cell proliferation in a wide range of cell types, and may also have an anti-apoptotic function. Transcription of this gene is highly inducible in response to many cytokines and hormones. In addition, the expression from some but not all inactive X chromosomes suggests that this gene inactivation is polymorphic in human females. This gene is located within intron 6 of the synapsin I gene and is transcribed in the opposite direction.

Background References

1. Guccini I et al. Senescence Reprogramming by TIMP1 Deficiency Promotes Prostate Cancer Metastasis. Cancer Cell. 2021 Jan

2. Schoeps B et al. TIMP1 Triggers Neutrophil Extracellular Trap Formation in Pancreatic Cancer. Cancer Res. 2021 Jul

Subcellular Location

Secreted.

UNIPROT

SwissProt: P01033 Human

Synonyms

Clgi antibody

Collagenase inhibitor antibody

Collagenase inhibitor, Human antibody

EPA antibody

EPO antibody

Erythroid Potentiating Activity antibody

Erythroid-potentiating activity antibody

Fibroblast collagenase inhibitor antibody

FLJ90373 antibody

HCI antibody

Expand

Images

  • Western blot analysis of TIMP1 on different lysates with Rabbit anti-TIMP1 antibody (<a href="/products/HA721741" style="font-weight: bold;text-decoration: underline;">HA721741</a>) at 1/1,000 dilution.<br /><br />Lane 1: HT-29 cell lysate<br />Lane 2: PANC-1 cell lysate<br />Lane 3: SW1990 cell lysate<br /><br />Lysates/proteins at 40 µg/Lane.<br /><br />Predicted band size: 23 kDa<br />Observed band size: 30 kDa<br /><br />Exposure time: 3 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721741" style="font-weight: bold;text-decoration: underline;">HA721741</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of TIMP1 on different lysates with Rabbit anti-TIMP1 antibody (HA721741) at 1/1,000 dilution.

    Lane 1: HT-29 cell lysate
    Lane 2: PANC-1 cell lysate
    Lane 3: SW1990 cell lysate

    Lysates/proteins at 40 µg/Lane.

    Predicted band size: 23 kDa
    Observed band size: 30 kDa

    Exposure time: 3 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721741) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-TIMP1 antibody (<a href="/products/HA721741" style="font-weight: bold;text-decoration: underline;">HA721741</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721741" style="font-weight: bold;text-decoration: underline;">HA721741</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human pancreas tissue with Rabbit anti-TIMP1 antibody (HA721741) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721741) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of HT-29 cells labeling TIMP1.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721741" style="font-weight: bold;text-decoration: underline;">HA721741</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HT-29 cells labeling TIMP1.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721741, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"