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HOXB4 Recombinant Rabbit Monoclonal Antibody [JE36-65]

Usd: 385

Specification

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Catalog# HA721739

HOXB4 Recombinant Rabbit Monoclonal Antibody [JE36-65]

Application

  • WB

  • IHC-P

  • FC

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

HOXB4 Recombinant Rabbit Monoclonal Antibody [JE36-65]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human HOXB4 aa 11-60 / 251.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB, IHC-P, FC

Molecular Weight

Predicted band size: 28 kDa

Positive Control

Jurkat cell lysate, HL-60 cell lysate, PC-12 cell lysate, mouse liver tissue lysate, mouse embryo cartilage tissue, HL-60.

Conjugation

unconjugated

Clone Number

JE36-65

RRID

AB_3072851

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:200

  • FC

  • 1:1,000

Target

Function

This gene is a member of the Antp homeobox family and encodes a nuclear protein with a homeobox DNA-binding domain. It is included in a cluster of homeobox B genes located on chromosome 17. The encoded protein functions as a sequence-specific transcription factor that is involved in development. Intracellular or ectopic expression of this protein expands hematopoietic stem and progenitor cells in vivo and in vitro, making it a potential candidate for therapeutic stem cell expansion.

Background References

1. Liang J et al. AKR1C3 and Its Transcription Factor HOXB4 Are Promising Diagnostic Biomarkers for Acute Myocardial Infarction. Front Cardiovasc Med. 2021 Sep

2. Wang L et al. HOXB4 Mis-Regulation Induced by Microcystin-LR and Correlated With Immune Infiltration Is Unfavorable to Colorectal Cancer Prognosis. Front Oncol. 2022 Feb

Subcellular Location

Nucleus.

UNIPROT

SwissProt: P17483 Human

SwissProt: P10284 Mouse

Entrez Gene: 497988 Rat

Synonyms

Homeo box 2F antibody

Homeo box B4 antibody

Homeobox 2F antibody

Homeobox B4 antibody

Homeobox protein Hox B4 antibody

Homeobox protein Hox-2.6 antibody

Homeobox protein Hox-2F antibody

Homeobox protein Hox-B4 antibody

Homeobox protein HoxB4 antibody

HOX 2 antibody

Expand

Images

  • Western blot analysis of HOXB4 on different lysates with Rabbit anti-HOXB4 antibody (<a href="/products/HA721739" style="font-weight: bold;text-decoration: underline;">HA721739</a>) at 1/1,000 dilution.<br /><br />Lane 1: Jurkat cell lysate<br />Lane 2: HL-60 cell lysate<br />Lane 3: PC-12 cell lysate<br />Lane 4: Mouse liver tissue lysate<br /><br />Lysates/proteins at 30 µg/Lane.<br /><br />Predicted band size: 28 kDa<br />Observed band size: 34 kDa<br /><br />Exposure time: 5 minutes;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721739" style="font-weight: bold;text-decoration: underline;">HA721739</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of HOXB4 on different lysates with Rabbit anti-HOXB4 antibody (HA721739) at 1/1,000 dilution.

    Lane 1: Jurkat cell lysate
    Lane 2: HL-60 cell lysate
    Lane 3: PC-12 cell lysate
    Lane 4: Mouse liver tissue lysate

    Lysates/proteins at 30 µg/Lane.

    Predicted band size: 28 kDa
    Observed band size: 34 kDa

    Exposure time: 5 minutes;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721739) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunohistochemical analysis of paraffin-embedded mouse embryo cartilage tissue with Rabbit anti-HOXB4 antibody (<a href="/products/HA721739" style="font-weight: bold;text-decoration: underline;">HA721739</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721739" style="font-weight: bold;text-decoration: underline;">HA721739</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse embryo cartilage tissue with Rabbit anti-HOXB4 antibody (HA721739) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with sodium citrate buffer (pH 6.0) (high pressure) for 2 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721739) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Flow cytometric analysis of HL-60 cells labeling HOXB4.<br /><br />Cells were fixed and permeabilized. Then stained with the primary antibody (<a href="/products/HA721739" style="font-weight: bold;text-decoration: underline;">HA721739</a>, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor&trade; 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (<a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

    Flow cytometric analysis of HL-60 cells labeling HOXB4.

    Cells were fixed and permeabilized. Then stained with the primary antibody (HA721739, 1μg/mL) (red) compared with Rabbit IgG Isotype Control (green). After incubation of the primary antibody at +4℃ for an hour, the cells were stained with a iFluor™ 488 conjugate-Goat anti-Rabbit IgG Secondary antibody (HA1121) at 1/1,000 dilution for 30 minutes at +4℃. Unlabelled sample was used as a control (cells without incubation with primary antibody; black).

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