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IL17A Receptor Recombinant Rabbit Monoclonal Antibody [JE35-89]

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Specification

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Catalog# HA721738

IL17A Receptor Recombinant Rabbit Monoclonal Antibody [JE35-89]

Application

  • WB

  • IF-Cell

Reactivity

  • Human

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

IL17A Receptor Recombinant Rabbit Monoclonal Antibody [JE35-89]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human IL17A Receptor aa 801-850 / 866.

Species Reactivity

Human

Validated Applications

WB, IF-Cell

Molecular Weight

Predicted band size: 96 kDa

Positive Control

THP-1 cell lysate, HeLa cell lysate, K-562 cell lysate, Jurkat cell lysate, Raji cell lysate, HL-60 cell lysate, HeLa.

Conjugation

unconjugated

Clone Number

JE35-89

RRID

AB_3072850

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000-1:2,000

  • IF-Cell

  • 1:100

Target

Function

Interleukin 17 receptor A, also known as IL17RA and CDw217 (cluster of differentiation w217), is a human gene. Interleukin 17A (IL17A)is a proinflammatory cytokine secreted by activated T-lymphocytes. It is a potent inducer of the maturation of CD34-positive hematopoietic precursors into neutrophils. The protein encoded by this gene (interleukin 17A receptor; IL17RA) is a ubiquitous type I membrane glycoprotein that binds with low affinity to interleukin 17A. Interleukin 17A and its receptor play a pathogenic role in many inflammatory and autoimmune diseases such as rheumatoid arthritis. Like other cytokine receptors, this receptor likely has a multimeric structure.

Background References

1. Nejman-Gryz P et al. The expression of IL17RA on sputum macrophages in asthma patients. Cytokine. 2021 Jul

2. Lauhkonen E et al. IL17RA variations showed no associations with post-bronchiolitis asthma or lung function. Pediatr Int. 2021 Feb

Subcellular Location

Cell membrane, Secreted.

UNIPROT

SwissProt: Q96F46 Human

Synonyms

CANDF5 antibody

CD217 antibody

CD217 antigen antibody

CDw217 antibody

CTLA8 antibody

HIL 17R antibody

hIL17R antibody

I17RA_HUMAN antibody

IL 17 receptor A antibody

IL 17 receptor antibody

Expand

Images

  • Western blot analysis of IL17A Receptor on different lysates with Rabbit anti-IL17A Receptor antibody (<a href="/products/HA721738" style="font-weight: bold;text-decoration: underline;">HA721738</a>) at 1/1,000 dilution.<br /><br />Lane 1: THP-1 cell lysate<br />Lane 2: HeLa cell lysate<br />Lane 3: K-562 cell lysate<br />Lane 4: Jurkat cell lysate<br />Lane 5: Raji cell lysate<br />Lane 6: HL-60 cell lysate<br /><br />Lysates/proteins at 25 µg/Lane.<br /><br />Predicted band size: 96 kDa<br />Observed band size: 160 kDa<br /><br />Exposure time: 30 seconds;<br /><br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721738" style="font-weight: bold;text-decoration: underline;">HA721738</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    Western blot analysis of IL17A Receptor on different lysates with Rabbit anti-IL17A Receptor antibody (HA721738) at 1/1,000 dilution.

    Lane 1: THP-1 cell lysate
    Lane 2: HeLa cell lysate
    Lane 3: K-562 cell lysate
    Lane 4: Jurkat cell lysate
    Lane 5: Raji cell lysate
    Lane 6: HL-60 cell lysate

    Lysates/proteins at 25 µg/Lane.

    Predicted band size: 96 kDa
    Observed band size: 160 kDa

    Exposure time: 30 seconds;

    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721738) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Knockdown (KD)</span><br /><br />Western blot analysis of IL17A Receptor on different lysates with Rabbit anti-IL17A Receptor antibody (<a href="/products/HA721738" style="font-weight: bold;text-decoration: underline;">HA721738</a>) at 1/2,000 dilution.<br /><br />Lane 1: HAP1-parental cell lysate<br />Lane 2: HAP1-IL17A Receptor KD cell lysate<br /><br />Lysates/proteins at 10 µg/Lane.<br /><br />Predicted band size: 96 kDa<br />Observed band size: 160 kDa<br /><br />Exposure time: 180 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721738" style="font-weight: bold;text-decoration: underline;">HA721738</a>) at 1/2,000 dilution was used in <a href="/products/K1803" style="font-weight: bold;text-decoration: underline;">K1803</a> at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Knockdown (KD)

    Western blot analysis of IL17A Receptor on different lysates with Rabbit anti-IL17A Receptor antibody (HA721738) at 1/2,000 dilution.

    Lane 1: HAP1-parental cell lysate
    Lane 2: HAP1-IL17A Receptor KD cell lysate

    Lysates/proteins at 10 µg/Lane.

    Predicted band size: 96 kDa
    Observed band size: 160 kDa

    Exposure time: 180 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721738) at 1/2,000 dilution was used in K1803 at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • Immunocytochemistry analysis of HeLa cells labeling IL17A Receptor with Rabbit anti-IL17A Receptor antibody (<a href="/products/HA721738" style="font-weight: bold;text-decoration: underline;">HA721738</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL17A Receptor antibody (<a href="/products/HA721738" style="font-weight: bold;text-decoration: underline;">HA721738</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/M1305-2" style="font-weight: bold;text-decoration: underline;">M1305-2</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of HeLa cells labeling IL17A Receptor with Rabbit anti-IL17A Receptor antibody (HA721738) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 20 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 5 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-IL17A Receptor antibody (HA721738) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (M1305-2, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation