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Phospho-PDGFR beta (Y1009) Recombinant Rabbit Monoclonal Antibody [JE46-72]

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Specification

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Catalog# HA721736

Phospho-PDGFR beta (Y1009) Recombinant Rabbit Monoclonal Antibody [JE46-72]

Application

  • WB

Reactivity

  • Human

  • Mouse

  • Rat

Conjugation

  • unconjugated

Overview

Product Name

Phospho-PDGFR beta (Y1009) Recombinant Rabbit Monoclonal Antibody [JE46-72]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic phosphopeptide corresponding to residues surrounding Tyr1009 of human PDGFR beta.

Species Reactivity

Human, Mouse, Rat

Validated Applications

WB

Molecular Weight

Predicted band size: 124 kDa

Positive Control

NIH/3T3 treated with 100ng/mL PDGF for 5 minutescell lysate, SH-SY5Y treated with 100ng/mL PDGF for 5 minutes cell lysate, C6 treated with 100ng/mL PDGF for 5 minutes cell lysate.

Conjugation

unconjugated

Clone Number

JE46-72

RRID

AB_3072848

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

Target

Function

The protein encoded by this gene is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer (PDGFB or PDGFD) or a heterodimer (PDGFA and PDGFB). This gene is essential for normal development of the cardiovascular system and aids in rearrangement of the actin cytoskeleton. This gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the ETV6 gene, results in chronic myeloproliferative disorder with eosinophilia.

Background References

1. Cui Z et al. Endothelial PDGF-BB/PDGFR-β signaling promotes osteoarthritis by enhancing angiogenesis-dependent abnormal subchondral bone formation. Bone Res. 2022 Aug

2. Hu G et al. PDGFR-β+ fibroblasts deteriorate survival in human solid tumors: a meta-analysis. Aging (Albany NY). 2021 May

Subcellular Location

Cell membrane, Cytoplasmic vesicle, Lysosome lumen.

UNIPROT

SwissProt: P09619 Human

SwissProt: P05622 Mouse

SwissProt: Q05030 Rat

Synonyms

Beta platelet derived growth factor receptor antibody

Beta-type platelet-derived growth factor receptor antibody

CD 140B antibody

CD140 antigen-like family member B antibody

CD140b antibody

CD140b antigen antibody

IBGC4 antibody

IMF1 antibody

JTK12 antibody

OTTHUMP00000160528 antibody

Expand

Images

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-PDGFR beta (Y1009) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y1009) antibody (<a href="/products/HA721736" style="font-weight: bold;text-decoration: underline;">HA721736</a>) at 1/1,000 dilution.<br /><br />Lane 1: NIH/3T3 cell lysate<br />Lane 2: NIH/3T3 treated with 100ng/mL PDGF for 5 minutescell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 124 kDa<br />Observed band size: 200 kDa (Glycosylation)<br /><br />Exposure time: 3 minutes;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721736" style="font-weight: bold;text-decoration: underline;">HA721736</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-PDGFR beta (Y1009) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y1009) antibody (HA721736) at 1/1,000 dilution.

    Lane 1: NIH/3T3 cell lysate
    Lane 2: NIH/3T3 treated with 100ng/mL PDGF for 5 minutescell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 124 kDa
    Observed band size: 200 kDa (Glycosylation)

    Exposure time: 3 minutes;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721736) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

  • <span style="font-weight: bold;">☑ Cell treatment (CT)</span><br /><br />Western blot analysis of Phospho-PDGFR beta (Y1009) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y1009) antibody (<a href="/products/HA721736" style="font-weight: bold;text-decoration: underline;">HA721736</a>) at 1/1,000 dilution.<br /><br />Lane 1: SH-SY5Y cell lysate<br />Lane 2: SH-SY5Y treated with 100ng/mL PDGF for 5 minutes cell lysate<br />Lane 3: C6 cell lysate<br />Lane 4: C6 treated with 100ng/mL PDGF for 5 minutes cell lysate<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 124 kDa<br />Observed band size: 200 kDa (Glycosylation)<br /><br />Exposure time: 25 seconds; ECL: K1801;<br />4-20% SDS-PAGE gel.<br /><br />Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (<a href="/products/HA721736" style="font-weight: bold;text-decoration: underline;">HA721736</a>) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (<a href="/products/HA1001" style="font-weight: bold;text-decoration: underline;">HA1001</a>) at 1/50,000 dilution was used for 1 hour at room temperature.

    ☑ Cell treatment (CT)

    Western blot analysis of Phospho-PDGFR beta (Y1009) on different lysates with Rabbit anti-Phospho-PDGFR beta (Y1009) antibody (HA721736) at 1/1,000 dilution.

    Lane 1: SH-SY5Y cell lysate
    Lane 2: SH-SY5Y treated with 100ng/mL PDGF for 5 minutes cell lysate
    Lane 3: C6 cell lysate
    Lane 4: C6 treated with 100ng/mL PDGF for 5 minutes cell lysate

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 124 kDa
    Observed band size: 200 kDa (Glycosylation)

    Exposure time: 25 seconds; ECL: K1801;
    4-20% SDS-PAGE gel.

    Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721736) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"