ErbB4 Recombinant Rabbit Monoclonal Antibody [JE59-43]
Catalog# HA721720
ErbB4 Recombinant Rabbit Monoclonal Antibody [JE59-43]
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WB
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Human
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Mouse
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Rat
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unconjugated
Overview
Product Name
ErbB4 Recombinant Rabbit Monoclonal Antibody [JE59-43]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Synthetic peptide within Human ErbB4 aa 1,231-1,280 / 1,308.
Species Reactivity
Human, Mouse, Rat
Validated Applications
WB
Molecular Weight
Predicted band size: 147 kDa
Positive Control
Mouse brain tissue lysate, rat brain tissue lysate.
Conjugation
unconjugated
Clone Number
JE59-43
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
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1:1,000
Target
Function
This gene is a member of the Tyr protein kinase family and the epidermal growth factor receptor subfamily. It encodes a single-pass type I membrane protein with multiple cysteine rich domains, a transmembrane domain, a tyrosine kinase domain, a phosphotidylinositol-3 kinase binding site and a PDZ domain binding motif. The protein binds to and is activated by neuregulins and other factors and induces a variety of cellular responses including mitogenesis and differentiation. Multiple proteolytic events allow for the release of a cytoplasmic fragment and an extracellular fragment. Mutations in this gene have been associated with cancer. Alternatively spliced variants which encode different protein isoforms have been described; however, not all variants have been fully characterized.
Background References
1. Segers VFM et al. The role of ErbB4 in cancer. Cell Oncol (Dordr). 2020 Jun
2. Lucas LM et al. The Yin and Yang of ERBB4: Tumor Suppressor and Oncoprotein. Pharmacol Rev. 2022 Jan
Subcellular Location
Cell membrane; Nucleus, Mitochondrion.
Synonyms
4ICD antibody
ALS19 antibody
Avian erythroblastic leukemia viral oncogene homolog 4 antibody
Avian erythroblastic leukemia viral v erb b2 oncogene homolog 4 antibody
E4ICD antibody
EC 2.7.10.1 antibody
ErbB 4 antibody
Erbb4 antibody
ERBB4 intracellular domain antibody
ERBB4_HUMAN antibody
ExpandImages
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Western blot analysis of ErbB4 on different lysates with Rabbit anti-ErbB4 antibody (HA721720) at 1/1,000 dilution.
Lane 1: Mouse brain tissue lysate
Lane 2: Rat brain tissue lysate
Lysates/proteins at 40 µg/Lane.
Predicted band size: 147 kDa
Observed band size: 180 kDa
Exposure time: 5 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721720) at 1/1,000 dilution was used in 5% NFDM/TBST at room temperature for 2 hours. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/100,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"