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Melanoma gp100 Recombinant Rabbit Monoclonal Antibody [JE42-47]

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Specification

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Catalog# HA721719

Melanoma gp100 Recombinant Rabbit Monoclonal Antibody [JE42-47]

Application

  • WB

  • IHC-P

  • IF-Cell

Reactivity

  • Human

  • Mouse

Conjugation

  • unconjugated

This product has been cited in peer reviewed publications, see list HERE

Overview

Product Name

Melanoma gp100 Recombinant Rabbit Monoclonal Antibody [JE42-47]

Antibody Type

Recombinant Rabbit monoclonal Antibody

Immunogen

Synthetic peptide within Human Melanoma gp100 aa 617-661 / 661.

Species Reactivity

Human, Mouse

Validated Applications

WB, IHC-P, IF-Cell

Molecular Weight

Predicted band size: 70 kDa

Positive Control

SK-MEL-28 cell lysates, human melanoma tissue, mouse testis tissue, SK-MEL-28.

Conjugation

unconjugated

Clone Number

JE42-47

RRID

AB_3072832

Product Features

Form

Liquid

Concentration

Storage Instructions

Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.

Storage Buffer

1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Isotype

IgG

Purification Method

Protein A affinity purified.

Application Dilution

  • WB

  • 1:1,000

  • IHC-P

  • 1:1,000

  • IF-Cell

  • 1:100

Target

Function

This gene encodes a melanocyte-specific type I transmembrane glycoprotein. The encoded protein is enriched in melanosomes, which are the melanin-producing organelles in melanocytes, and plays an essential role in the structural organization of premelanosomes. This protein is involved in generating internal matrix fibers that define the transition from Stage I to Stage II melanosomes. This protein undergoes a complex pattern of prosttranslational processing and modification that is essential to the proper functioning of the protein. A secreted form of this protein that is released by proteolytic ectodomain shedding may be used as a melanoma-specific serum marker. Alternate splicing results in multiple transcript variants.

Background References

1. AlAbdi L et al. PMEL is mutated in oculocutaneous albinism. Hum Genet. 2023 Jan

2. Kimura S et al. PMEL p.Leu18del dilutes coat color of Kumamoto sub-breed of Japanese Brown cattle. BMC Genomics. 2022 Oct

Subcellular Location

Endoplasmic reticulum membrane, Golgi apparatus, cis-Golgi network membrane, Endosome, multivesicular body, Melanosome, Extracellular vesicle, Secreted.

UNIPROT

SwissProt: P40967 Human

SwissProt: Q60696 Mouse

Synonyms

95 kDa melanocyte specific secreted glycoprotein antibody

95 kDa melanocyte-specific secreted glycoprotein antibody

D12S53E antibody

gp100 antibody

M-beta antibody

ME20 antibody

ME20 M/ME20 S antibody

ME20-M antibody

ME20-S antibody

ME20M antibody

Expand

Images

  • Immunohistochemical analysis of paraffin-embedded human melanoma tissue with Rabbit anti-Melanoma gp100 antibody (<a href="/products/HA721719" style="font-weight: bold;text-decoration: underline;">HA721719</a>) at 1/1,000 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721719" style="font-weight: bold;text-decoration: underline;">HA721719</a>) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded human melanoma tissue with Rabbit anti-Melanoma gp100 antibody (HA721719) at 1/1,000 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721719) at 1/1,000 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Melanoma gp100 antibody (<a href="/products/HA721719" style="font-weight: bold;text-decoration: underline;">HA721719</a>) at 1/200 dilution.<br /><br />The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH<sub>2</sub>O and PBS, and then probed with the primary antibody (<a href="/products/HA721719" style="font-weight: bold;text-decoration: underline;">HA721719</a>) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

    Immunohistochemical analysis of paraffin-embedded mouse testis tissue with Rabbit anti-Melanoma gp100 antibody (HA721719) at 1/200 dilution.

    The section was pre-treated using heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0) for 20 minutes. The tissues were blocked in 1% BSA for 20 minutes at room temperature, washed with ddH2O and PBS, and then probed with the primary antibody (HA721719) at 1/200 dilution for 1 hour at room temperature. The detection was performed using an HRP conjugated compact polymer system. DAB was used as the chromogen. Tissues were counterstained with hematoxylin and mounted with DPX.

  • Immunocytochemistry analysis of SK-MEL-28 cells labeling Melanoma gp100 with Rabbit anti-Melanoma gp100 antibody (<a href="/products/HA721719" style="font-weight: bold;text-decoration: underline;">HA721719</a>) at 1/100 dilution.<br /><br />Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Melanoma gp100 antibody (<a href="/products/HA721719" style="font-weight: bold;text-decoration: underline;">HA721719</a>) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor&trade; 488, <a href="/products/HA1121" style="font-weight: bold;text-decoration: underline;">HA1121</a>) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.<br /><br />Beta tubulin (<a href="/products/HA601187" style="font-weight: bold;text-decoration: underline;">HA601187</a>, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor&trade; 594, <a href="/products/HA1126" style="font-weight: bold;text-decoration: underline;">HA1126</a>) was used as the secondary antibody at 1/1,000 dilution.

    Immunocytochemistry analysis of SK-MEL-28 cells labeling Melanoma gp100 with Rabbit anti-Melanoma gp100 antibody (HA721719) at 1/100 dilution.

    Cells were fixed in 4% paraformaldehyde for 15 minutes at room temperature, permeabilized with 0.1% Triton X-100 in PBS for 15 minutes at room temperature, then blocked with 1% BSA in 10% negative goat serum for 1 hour at room temperature. Cells were then incubated with Rabbit anti-Melanoma gp100 antibody (HA721719) at 1/100 dilution in 1% BSA in PBST overnight at 4 ℃. Goat Anti-Rabbit IgG H&L (iFluor™ 488, HA1121) was used as the secondary antibody at 1/1,000 dilution. PBS instead of the primary antibody was used as the secondary antibody only control. Nuclear DNA was labelled in blue with DAPI.

    Beta tubulin (HA601187, red) was stained at 1/100 dilution overnight at +4℃. Goat Anti-Mouse IgG H&L (iFluor™ 594, HA1126) was used as the secondary antibody at 1/1,000 dilution.

  • Western blot analysis of Melanoma gp100 on SK-MEL-28 cell lysates with Rabbit anti-Melanoma gp100 antibody (<a href="/products/HA721719" style="font-weight: bold;text-decoration: underline;">HA721719</a>) at 1/1,000 dilution.<br /><br />Lysates/proteins at 20 µg/Lane.<br /><br />Predicted band size: 70 kDa<br />Observed band size: 100 kDa<br /><br />Exposure time: 2 minutes;

    Western blot analysis of Melanoma gp100 on SK-MEL-28 cell lysates with Rabbit anti-Melanoma gp100 antibody (HA721719) at 1/1,000 dilution.

    Lysates/proteins at 20 µg/Lane.

    Predicted band size: 70 kDa
    Observed band size: 100 kDa

    Exposure time: 2 minutes;

Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"

Citation