PTH1R Recombinant Rabbit Monoclonal Antibody [JE36-10]
Catalog# HA721716
PTH1R Recombinant Rabbit Monoclonal Antibody [JE36-10]
-
WB
-
Human
-
unconjugated
Overview
Product Name
PTH1R Recombinant Rabbit Monoclonal Antibody [JE36-10]
Antibody Type
Recombinant Rabbit monoclonal Antibody
Immunogen
Recombinant protein within Human PTH1R aa 39-188 / 593.
Species Reactivity
Human
Validated Applications
WB
Molecular Weight
Predicted band size: 66 kDa
Positive Control
HeLa cell lysate, HepG2 cell lysate, MCF7 cell lysate, THP-1 cell lysate.
Conjugation
unconjugated
Clone Number
JE36-10
RRID
Product Features
Form
Liquid
Concentration
Storage Instructions
Shipped at 4℃. Store at +4℃ short term (1-2 weeks). Store at -20℃ long term.
Storage Buffer
1*TBS (pH7.4), 0.05% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Isotype
IgG
Purification Method
Protein A affinity purified.
Application Dilution
-
WB
-
1:1,000
Target
Function
The protein encoded by this gene is a member of the G-protein coupled receptor family 2. This protein is a receptor for parathyroid hormone (PTH) and for parathyroid hormone-like hormone (PTHLH). The activity of this receptor is mediated by G proteins which activate adenylyl cyclase and also a phosphatidylinositol-calcium second messenger system. Defects in this receptor are known to be the cause of Jansen's metaphyseal chondrodysplasia (JMC), chondrodysplasia Blomstrand type (BOCD), as well as enchodromatosis. Two transcript variants encoding the same protein have been found for this gene.
Background References
1. Martin TJ. PTH1R Actions on Bone Using the cAMP/Protein Kinase A Pathway. Front Endocrinol (Lausanne). 2022 Jan
2. Wang MW et al. Activation of PTH1R alleviates epididymitis and orchitis through Gq and β-arrestin-1 pathways. Proc Natl Acad Sci U S A. 2021 Nov
Subcellular Location
Cell membrane.
UNIPROT
Synonyms
MGC138426 antibody
MGC138452 antibody
Parathyroid hormone 1 receptor antibody
Parathyroid hormone/parathyroid hormone related peptide receptor antibody
Parathyroid hormone/parathyroid hormone related protein receptor antibody
Parathyroid hormone/parathyroid hormone-related peptide receptor antibody
PTH receptor antibody
PTH/PTHr receptor antibody
PTH/PTHrP receptor antibody
PTH/PTHrP type I receptor antibody
ExpandImages
-
Western blot analysis of PTH1R on different lysates with Rabbit anti-PTH1R antibody (HA721716) at 1/1,000 dilution.
Lane 1: HeLa cell lysate
Lane 2: HepG2 cell lysate
Lane 3: MCF7 cell lysate
Lane 4: THP-1 cell lysate
Lysates/proteins at 20 µg/Lane.
Predicted band size: 66 kDa
Observed band size: 66 kDa
Exposure time: 3 minutes;
4-20% SDS-PAGE gel.
Proteins were transferred to a PVDF membrane and blocked with 5% NFDM/TBST for 1 hour at room temperature. The primary antibody (HA721716) at 1/1,000 dilution was used in 5% NFDM/TBST at 4℃ overnight. Goat Anti-Rabbit IgG - HRP Secondary Antibody (HA1001) at 1/50,000 dilution was used for 1 hour at room temperature.
Please note: All products are "FOR RESEARCH USE ONLY AND ARE NOT INTENDED FOR DIAGNOSTIC OR THERAPEUTIC USE"